Geranylgeraniol Induces PPARγ Expression and Enhances the Biological Effects of a PPARγ Agonist in Adipocyte Lineage Cells
| Autor: | Yukiyo Shigeyama-Tada, Atsuko Nakamichi, Yuya Muramatsu, Shoichiro Kokabu, Kazuma Yasuda, Nana Takakura, Chihiro Nakatomi, Mariko Urata, Kou Matsuo, Takeshi Kaneuji, Takuma Matsubara, Makoto Tsuboi, Min Zhang, William N. Addison |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.drug_class General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Mice Geranylgeraniol Adipocyte Lipid droplet 3T3-L1 Cells medicine Adipocytes Oil Red O Animals Thiazolidinedione Receptor Pharmacology Fibroblasts Cell biology PPAR gamma 030104 developmental biology chemistry Gene Expression Regulation Adipogenesis Diterpenes Hydroxymethylglutaryl-CoA Reductase Inhibitors Rosiglitazone medicine.drug Research Article |
| Popis: | Background The global incidence of diabetes mellitus (DM) has risen precipitously, even in middle- and low-income countries. Peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in the control of cellular glucose metabolism. Activation of PPARγ beneficially results in increased insulin sensitivity. However, the expression of PPARγ is reduced by obesity and several nutritional factors. Here we examined the effect of geranylgeraniol (GGOH), a bioactive compound found naturally in fruits, vegetables, and grains, on the expression and activation of PPARγ. Materials and methods C3H10T1/2 mouse embryonic fibroblasts and 3T3-L1 pre-adipocytes were used as in vitro models of adipocyte differentiation and function. Quantitative reverse-transcriptase polymerase chain reaction, western blotting, Oil Red O staining, and luciferase assay were performed to respectively assess mRNA expression, protein levels, lipid droplet formation and transcriptional activity. Results GGOH increased the expression of PPARγ in adipocyte lineage cells. GGOH also enhanced adipogenesis induced by rosiglitazone, a thiazolidinedione class PPARγ agonist. Conclusion GGOH induces PPARγ expression and enhances the biological effects of a PPARγ agonist in adipocyte lineage cells. |
| Databáze: | OpenAIRE |
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