Detection of the oil-producing microalga Botryococcus braunii in natural freshwater environments by targeting the hydrocarbon biosynthesis gene SSL-3
| Autor: | Naru Takayama, Koji Kawamura, Takuya Hara, Gumiri Sulmin, Ardianor, Yukio Komai, Kotaro Hirano, Rudy Agung Nugroho, Shigeru Okada, Hendrik Segah |
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| Rok vydání: | 2019 |
| Předmět: |
Water microbiology
0301 basic medicine Pcr assay lcsh:Medicine Fresh Water Diurnal change Real-Time Polymerase Chain Reaction Article Microbial ecology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Japan Biosynthesis Dry weight Chlorophyta Botany Microalgae Botryococcus braunii lcsh:Science Gene Ecosystem Phylogeny chemistry.chemical_classification Tropical Climate Multidisciplinary Geography biology lcsh:R fungi Algal Proteins Gene Amplification Biofuel feedstock biology.organism_classification Hydrocarbons Biosynthetic Pathways 030104 developmental biology Hydrocarbon chemistry Indonesia Biofuels lcsh:Q Oils 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-53619-y |
| Popis: | The green microalga Botryococcus braunii produces hydrocarbon oils at 25–75% of its dry weight and is a promising source of biofuel feedstock. Few studies have examined this species’ ecology in natural habitats, and few wild genetic resources have been collected due to difficulties caused by its low abundance in nature. This study aimed to develop a real-time PCR assay for specific detection and quantification of this alga in natural environments and to quantify spatiotemporal variations of wild B. braunii populations in a tropical pond. We designed PCR primers toward the hydrocarbon biosynthesis gene SSL-3 and examined amplification specificity and PCR efficiency with 70 wild strains newly isolated from various environments. The results demonstrated that this PCR assay specifically amplified B. braunii DNA, especially that of B-race strains, and can be widely used to detect wild B. braunii strains in temperate and tropical habitats. Field-testing in a tropical pond suggested a diurnal change in the abundance of B. braunii in surface water and found B. braunii not only in surface water, but also at 1–1.5 m deep and in bottom sediments. This method can contribute to efficient genetic resource exploitations and may also help elucidate the unknown ecology of B. braunii. |
| Databáze: | OpenAIRE |
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