Efficient Differentiation of Mouse Embryonic Stem Cells into Insulin-Producing Cells
| Autor: | Szu-Hsiu Liu, Lain-Tze Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Niacinamide
medicine.medical_specialty lcsh:Internal medicine lcsh:Specialties of internal medicine Endocrinology Diabetes and Metabolism medicine.medical_treatment Cellular differentiation lcsh:Medicine Leukemia Inhibitory Factor lcsh:Diseases of the endocrine glands. Clinical endocrinology Embryo Culture Techniques Cell therapy Mice Laminin lcsh:RC581-951 Insulin-Secreting Cells Internal medicine Insulin Secretion medicine Animals Humans Insulin lcsh:RC31-1245 Embryonic Stem Cells Progenitor lcsh:RC648-665 biology Methodology Report Endoderm lcsh:R Cell Differentiation General Medicine Fibroblasts Embryonic stem cell Cell biology Glucose Phenotype Endocrinology medicine.anatomical_structure Microscopy Fluorescence biology.protein Definitive endoderm |
| Zdroj: | Experimental Diabetes Research, Vol 2012 (2012) Experimental Diabetes Research |
| ISSN: | 1687-5303 1687-5214 |
| Popis: | Embryonic stem (ES) cells are a potential source of a variety of differentiated cells for cell therapy, drug discovery, and toxicology screening. Here, we present an efficacy strategy for the differentiation of mouse ES cells into insulin-producing cells (IPCs) by a two-step differentiation protocol comprising of (i) the formation of definitive endoderm in monolayer culture by activin A, and (ii) this monolayer endoderm being induced to differentiate into IPCs by nicotinamide, insulin, and laminin. Differentiated cells can be obtained within approximately 7 days. The differentiation IPCs combined application of RT-PCR, ELISA, and immunofluorescence to characterize phenotypic and functional properties. In our study, we demonstrated that IPCs produced pancreatic transcription factors, endocrine progenitor marker, definitive endoderm, pancreaticβ-cell markers, and Langerhansαandδcells. The IPCs released insulin in a manner that was dose dependent upon the amount of glucose added. These techniques may be able to be applied to human ES cells, which would have very important ramifications for treating human disease. |
| Databáze: | OpenAIRE |
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