Bone Morphogenetic Protein-2 Plasmid DNA as a Substitute for Bone Morphogenetic Protein-2 Protein in Bone Tissue Engineering
| Autor: | Yvonne J.M van der Helm, Wouter J.A. Dhert, Fiona Wegman, Jacqueline Alblas, F. Cumhur Oner |
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| Rok vydání: | 2013 |
| Předmět: |
Bone Regeneration
animal structures Alginates Genetic enhancement Biomedical Engineering Bone Morphogenetic Protein 2 Bioengineering Gene delivery Biochemistry Regenerative medicine Bone morphogenetic protein 2 Bone and Bones Biomaterials Mice Glucuronic Acid Tissue engineering Plasmid dna Animals Humans Bone regeneration Tissue Engineering Chemistry Goats Hexuronic Acids Gene Transfer Techniques Genetic Therapy Cell biology Bone morphogenetic protein 7 embryonic structures Plasmids Biomedical engineering |
| Zdroj: | Tissue Engineering Part A. 19:2686-2692 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2012.0569 |
| Popis: | Bone regeneration is one of the focus points in the field of regenerative medicine. A well-known stimulus of bone formation is bone morphogenetic protein-2 (BMP-2), which has already been extensively used in clinical applications. However, due to a short half-life, supraphysiological doses are applied resulting in severe side effects such as ectopic bone formation or even loss of bone. We compared the effectivity of transient BMP-2 gene delivery with the BMP-2 protein at clinical (high) and physiological (low) doses by subcutaneous implantation of alginate-based constructs in mice. After 6 weeks of implantation, both the protein laden constructs and BMP-2 plasmid DNA-based constructs showed similar early bone onset and elevated bone formation compared to controls without any BMP-2 added. We found no differences in efficiency by using BMP-2 plasmid DNA or any of the BMP-2 protein dosages. Therefore, we conclude that BMP-2 plasmid DNA-based gene therapy in alginate is a promising new strategy for BMP-2 administration for bone (re)generation. |
| Databáze: | OpenAIRE |
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