Ternary Hybrid γ-Fe2O3/CrVI/Amine Oxidase Nanostructure for Electrochemical Sensing: Application for Polyamine Detection in Tumor Tissue
| Autor: | Radek Zboril, Ondrej Malina, Emanuela Bonaiuto, Alberto Macone, Fabio Vianello, Silvano Fasolato, Elisabetta Sconcerle, Petr Jakubec, Giovanni Miotto, Gabriella Salviulo, Enzo Agostinelli, Massimiliano Magro, Milo Terzo, Rina Venerando, Davide Baratella |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Amine oxidase
Carcinoma Hepatocellular Surface Properties Inorganic chemistry enzymes Nanoparticle Biosensing Techniques 02 engineering and technology 010402 general chemistry Electrochemistry Electrocatalyst Ferric Compounds 01 natural sciences Catalysis chemistry.chemical_compound Neoplasms nanostructures Polyamines Animals Humans cancer biosensors chromium Particle Size Hydrogen peroxide Magnetic Phenomena Liver Neoplasms Organic Chemistry Chemistry (all) Electrochemical Techniques General Chemistry Enzymes Immobilized 021001 nanoscience & nanotechnology 0104 chemical sciences Nanomedicine chemistry Nanoparticles Cattle Oxidoreductases 0210 nano-technology Polyamine Oxidation-Reduction Biosensor Nuclear chemistry |
| Popis: | Dichromate binds to surface-active maghemite nanoparticles (SAMNs) to form a stable core-shell nanostructures (SAMN@Cr(VI) ). The hybrid was characterized by Mössbauer spectroscopy, high-angle annular dark-field imaging, electron energy-loss spectroscopy, and electrochemical techniques, which revealed a strong interaction of dichromate with the nanoparticle surface. Electrochemical characterization showed lower charge-transfer resistance, better electrochemical performance, and more reversible electrochemical behavior with respect to naked SAMNs. Moreover, SAMN@Cr(VI) is an excellent electrocatalyst for hydrogen peroxide reduction. Furthermore, an enzyme, namely, bovine serum amine oxidase (BSAO: EC 1.4.3.6), was immobilized on SAMN@Cr(VI) by self-assembly to give a ternary hybrid nanostructured catalyst for polyamine oxidation (SAMN@Cr(VI) -BSAO). SAMN@Cr(VI) -BSAO was applied for the development of a reagentless, fast, inexpensive, and interference-free polyamine biosensor, which was successfully exploited for the discrimination of tumorous tissue from healthy tissue in human crude liver extracts. |
| Databáze: | OpenAIRE |
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