Homeostatic and Circadian Contribution to EEG and Molecular State Variables of Sleep Regulation
| Autor: | Paul Franken, Stéphane Dorsaz, Valérie Mongrain, Thomas Curie, Yann Emmenegger, Géraldine M. Mang |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Gene Expression Electroencephalography Biology Homeostatic Process Mice Mice Inbred AKR 03 medical and health sciences Prosencephalon 0302 clinical medicine Homer Scaffolding Proteins Physiology (medical) Internal medicine medicine Animals Homeostasis Circadian rhythm 030304 developmental biology 0303 health sciences medicine.diagnostic_test Period Circadian Proteins Sleep in non-human animals Circadian Rhythm DNA-Binding Proteins Mice Inbred C57BL CLOCK PER2 Disease Models Animal Sleep deprivation Editorial Endocrinology Sleep Deprivation Neurology (clinical) medicine.symptom Carrier Proteins Sleep 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Sleep. 36:311-323 |
| ISSN: | 1550-9109 0161-8105 |
| DOI: | 10.5665/sleep.2440 |
| Popis: | Study Objectives: Besides their well-established role in circadian rhythms, our findings that the forebrain expression of the clock-genes Per2 and Dbp increases and decreases, respectively, in relation to time spent awake suggest they also play a role in the homeostatic aspect of sleep regulation. Here, we determined whether time of day modulates the effects of elevated sleep pressure on clock-gene expression. Time of day effects were assessed also for recognized electrophysiological (EEG delta power) and molecular (Homer1a) markers of sleep homeostasis. Design: EEG and qPCR data were obtained for baseline and recovery from 6-h sleep deprivation starting at ZT0, -6, -12, or -18. Setting: Mouse sleep laboratory. Participants: Male mice. Interventions: Sleep deprivation. Results: The sleep-deprivation induced changes in Per2 and Dbp expression importantly varied with time of day, such that Per2 could even decrease during sleep deprivations occurring at the decreasing phase in baseline. Dbp showed similar, albeit opposite dynamics. These unexpected results could be reliably predicted assuming that these transcripts behave according to a driven damped harmonic oscillator. As expected, the sleep-wake distribution accounted for a large degree of the changes in EEG delta power and Homer1a. Nevertheless, the sleep deprivation-induced increase in delta power varied also with time of day with higher than expected levels when recovery sleep started at dark onset. Conclusions: Per2 and delta power are widely used as exclusive state variables of the circadian and homeostatic process, respectively. Our findings demonstrate a considerable cross-talk between these two processes. As Per2 in the brain responds to both sleep loss and time of day, this molecule is well positioned to keep track of and to anticipate homeostatic sleep need. |
| Databáze: | OpenAIRE |
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