Cell‐free culture supernatant of Lactobacillus curvatus Wikim38 inhibits RANKL‐induced osteoclast differentiation and ameliorates bone loss in ovariectomized mice
| Autor: | Ji-Yeon Park, Dong-Yeon Kim, Jae-Hun Ahn, Jong-Hwan Park, Joo-Hee Choi, Ji-Yoon Chang, Tae-Sung Lee, Joon-Suk Park, Dong-Kyu Kim, Ah-Ra Jang |
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| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Osteoporosis Osteoclasts Inhibitory postsynaptic potential Applied Microbiology and Biotechnology Bone resorption Mice Downregulation and upregulation Osteoclast Internal medicine medicine Animals Bone Resorption Bone mineral biology Chemistry NF-kappa B Cell Differentiation medicine.disease Lactobacillus Endocrinology medicine.anatomical_structure RANKL Ovariectomized rat biology.protein Female |
| Zdroj: | Letters in Applied Microbiology. 73:383-391 |
| ISSN: | 1472-765X 0266-8254 |
| Popis: | The present study was conducted to investigate the inhibitory effects of the cell-free culture supernatant of L. curvatus Wikim 38 (LC38-CS) on RANKL-induced osteoclast differentiation and bone loss in a mice model of ovariectomy-induced post-menopausal osteoporosis. LC38-CS inhibited the RANKL-induced differentiation of bone marrow-derived macrophages (BMDMs) into osteoclasts in a dose-dependent manner. F-actin ring formation and bone resorption were also reduced by LC38-CS treatment of RANKL-treated BMDMs. In addition, LC38-CS decreased the RANKL-induced activation of the TRAF6/NF-κB/MAPKs axis at the early stage and the expression of osteoclastogenesis-related genes in BMDMs treated with RANKL. PRMT1 and ADMA levels, new biomarkers for osteoclastogenesis, were decreased by LC38-CS treatment. The administration of LC38-CS increased bone volume and bone mineral density (BMD) in ovariectomized (OVX) mice in μ-CT analysis. These findings suggest that LC38-CS inhibited RANKL-induced osteoclast differentiation via the downregulation of molecular mechanisms and exerted anti-osteoporotic effects. |
| Databáze: | OpenAIRE |
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