Transcriptome analysis of human monocytic cells infected with Burkholderia species and exploration of pentraxin-3 as part of the innate immune response against the organisms
| Autor: | Henry V. Baker, Maria Cecilia Lopez, Eric R. Lafontaine, Sophie A. Aschenbroich, Robert J. Hogan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
lcsh:Internal medicine lcsh:QH426-470 Burkholderia Burkholderia thailandensis Pattern recognition receptor Burkholderia mallei Antibodies Monocytes Microbiology Cell Line Transcriptome 03 medical and health sciences 0302 clinical medicine Immune system Phagocytosis Opsonophagocytosis Genetics Humans lcsh:RC31-1245 Intracellular survival Genetics (clinical) PTX3 Innate immune system Microbial Viability biology Intracellular parasite Gene Expression Profiling Complement System Proteins Opsonin Proteins biology.organism_classification Immunity Innate Up-Regulation lcsh:Genetics Serum Amyloid P-Component 030104 developmental biology C-Reactive Protein 030220 oncology & carcinogenesis Human monocytes Protein Binding Research Article |
| Zdroj: | BMC Medical Genomics BMC Medical Genomics, Vol 12, Iss 1, Pp 1-16 (2019) |
| ISSN: | 1755-8794 |
| Popis: | Background Burkholderia mallei (Bm) is a facultative intracellular bacterial pathogen causing highly-fatal glanders in solipeds and humans. The ability of Bm to thrive intracellularly is thought to be related to exploitation of host immune response-related genes and pathways. Relatively little is known of the molecular strategies employed by this pathogen to modulate these pathways and evade intracellular killing. This manuscript seeks to fill gaps in the understanding of the interface between Bm and innate immunity by examining gene expression changes during infection of host monocytes. Methods The transcriptome of Bm-infected human Mono Mac-6 (MM6) monocytes was profiled on Affymetrix Human Transcriptome GeneChips 2.0. Gene expression changes in Bm-infected monocytes were compared to those of Burkholderia thailandensis (Bt)-infected monocytes and to uninfected monocytes. The resulting dataset was normalized using Robust Multichip Average and subjected to statistical analyses employing a univariate F test with a random variance model. Differentially expressed genes significant at p |
| Databáze: | OpenAIRE |
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