Tumor-associated macrophage infiltration is highly associated with PD-L1 expression in gastric adenocarcinoma
| Autor: | Randy L. Johnson, Wayne L. Hofstetter, Arlene M. Correa, Shumei Song, Xiaochuan Dong, Akihiro Suzuki, Jaffer A. Ajani, Kazuto Harada, Zhenning Wang, Yan Xu, Koyu Suzuki, Hisashi Onodera, Kazuki Sudo, Jeannelyn S. Estrella |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cancer Research Pathology medicine.medical_specialty Tumor-associated macrophage Adenocarcinoma B7-H1 Antigen 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms Tumor Microenvironment Medicine Humans Aged Retrospective Studies Tumor microenvironment Tissue microarray business.industry CD68 Macrophages Gastroenterology General Medicine Middle Aged medicine.disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Immunohistochemistry Female business CD163 |
| Zdroj: | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association. 21(1) |
| ISSN: | 1436-3305 |
| Popis: | Programmed death ligand 1 (PD-L1) is a key protein upregulated by tumor cells to suppress immune responses. Tumor-associated macrophages (TAMs) play a major role in this immunosuppression, but the relationship between PD-L1 expression and TAMs remains unclear in gastric adenocarcinoma (GAC). We simultaneously examined expression of PD-L1 and TAMs in GAC. We performed immunohistochemical staining for PD-L1, CD68 (pan-macrophage), and CD163 (M2-like macrophage) in 217 GAC samples using a tissue microarray. Expression of PD-L1 and CD68- and CD163-positive cells was evaluated using the Cytoplasmic V2.0 algorithm in Aperio ImageScope software, and logistic regression analysis was used to compare expression patterns between groups. Thirty-one samples (14%) were positive for PD-L1 expression. The mean (± standard error) rates of infiltration were 6.83 ± 0.38% for CD68-positive cells and 6.16 ± 0.29% for CD163-positive cells. The mean rate of CD163-positive cell infiltration was significantly higher in diffuse GAC than in intestinal GAC (diffuse n = 111, 6.91%; intestinal n = 91, 5.26%; p = 0.006), but the mean rate of CD68-positive cell infiltration was similar between these types (p = 0.38). The mean infiltration rates of CD68- and CD163-positive cells in PD-L1-positive GAC were significantly higher than in PD-L1-negative GAC (CD68 p = 0.0002; CD163 p |
| Databáze: | OpenAIRE |
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