Comparative genomics of epidemic versus sporadic Staphylococcus aureus strains does not reveal molecular markers for epidemicity
| Autor: | Dominique S. Blanc, Damian C. Melles, Patrice Francois, Margreet C. Vos, G. Kuhn, Jacques Schrenzel, Antoine Huyghe, Alex van Belkum, Giorgio Zanetti, Paul M. Dunman, Thibaud Koessler |
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| Přispěvatelé: | Medical Microbiology & Infectious Diseases |
| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Cross Infection/epidemiology Staphylococcus aureus Biology Methicillin Resistance/genetics medicine.disease_cause Microbiology Analysis of molecular variance Genome Disease Outbreaks Genome Bacterial/drug effects Genetics medicine Humans ORFS Allele Molecular Biology Ecology Evolution Behavior and Systematics Oligonucleotide Array Sequence Analysis Comparative genomics ddc:616 Staphylococcus aureus/ classification/drug effects/ genetics Cross Infection Comparative Genomic Hybridization Molecular Epidemiology Strain (biology) Genomics Staphylococcal Infections Staphylococcal Infections/ epidemiology Open reading frame Infectious Diseases Methicillin Resistance Genome Bacterial |
| Zdroj: | Infection, Genetics and Evolution, 10(1), 89-96. Elsevier Infection, Genetics and Evolution, Vol. 10, No 1 (2010) pp. 89-96 |
| ISSN: | 1567-1348 |
| Popis: | Staphylococcus aureus, especially when it is methicillin resistant, has been recognised as a major cause of nosocomial and community-acquired infections. It has also been shown that certain strains were able to cause clonal epidemics whereas others showed a more incidental occurrence. On the basis of this behavioural distinction, a genetic feature underlying this difference in epidemicity can be assumed. Understanding the difference will not only contribute to the development of markers for the identification of epidemic strains but will also shed light on the evolution of clones. Genomes of strains from two independent collections (n = 18 and n = 10 strains) were analysed. Both collections were composed of carefully selected, genetically diverse strains with clinically well-defined epidemic and sporadic behaviour. Comparative genome hybridisation (CGH) was performed using an Agilent array for one collection (up to 11 probes per open reading frame - ORF), and an Affymetrix array for the other(up to 30 probes per ORF). Presence and absence information of probe homologues and ORFs was taken for analysis of molecular variance (AMOVA) at the strain and behaviour levels. Not a single probe showed 100% concordant differences between epidemic and sporadic strains. Moreover, probe differences between groups were always smaller than those within groups. This was also true, when the analysis was focussed on presence versus absence of ORF's or when probe information was transformed into allelic profiles. These findings present strong evidence against the presence or absence of a single common specific genetic factor differentiating epidemic from sporadic S. aureus clones. (C) 2009 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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