Effect of Nerve Growth Factor on Osseointegration of Titanium Implants in Type 2 Diabetic Rats
| Autor: | Ryuji Yamamoto, Chikahiro Ohkubo, Jing Zhang, Mai Shirai, Yasuo Yamakoshi, Shinichiro Oida, Jianyu Zeng |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Population Urology Bone and Bones Osseointegration Diabetes Mellitus Experimental Random Allocation 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Bone-Implant Interface Nerve Growth Factor medicine Animals In patient Rats Wistar education Saline Dental Implants Titanium 030222 orthopedics education.field_of_study Tibia business.industry Dental Implantation Endosseous Type 2 Diabetes Mellitus 030206 dentistry General Medicine medicine.disease Rats Surgery Nerve growth factor Diabetes Mellitus Type 2 Implant Oral Surgery business |
| Zdroj: | The International Journal of Oral & Maxillofacial Implants. :1189-1194 |
| ISSN: | 0882-2786 |
| Popis: | Purpose: Compared with the general population, a poorer quality of bone-implant osseointegration occurs and at a higher failure rate in patients with type 2 diabetes mellitus. The aim of this study was to analyze the effects of local injection of nerve growth factor at the bone-implant interface after implantation in type 2 diabetic rats. Materials and Methods: Goto-Kakizaki (GK) rats (n = 30) were used as a model of type 2 diabetes mellitus, and Wistar rats were used as a control (n = 15). GK rats were assigned randomly into two groups (n = 15/group): the diabetes mellitus group (saline only) and the nerve growth factor group (received nerve growth factor treatment). One titanium implant was placed in each rat's left tibia. Immediately postoperatively, nerve growth factor group rats were injected with nerve growth factor (0.4 μg/ day) intramuscularly around the implant, daily for 7 days. Diabetes mellitus and control group rats received normal saline in an identical manner. Rats were sacrificed at 2, 4, and 8 weeks following implant surgery. Results: Traditional light and confocal laser scanning microscopy were used on nondecalcified sections to investigate fluorochrome labeling changes and histologic features of bone adjoining the implants. Bone-toimplant contact and bone volume percentage in the diabetes mellitus group were significantly less than in the control and nerve growth factor groups, with no statistically significant differences between the control and nerve growth factor groups. Confocal laser scanning microscopy showed a significant increase in marked bone around the nerve growth factor group implant at 4 weeks (P < .01) and 8 weeks (P < .05) compared with the diabetes mellitus group. Conclusion: This study showed that local injection of nerve growth factor could improve implant-bone osseointegration in diabetic rats and may have important clinical implications. |
| Databáze: | OpenAIRE |
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