Surveillance of transmitted HIV drug resistance among women attending antenatal clinics in Dar es Salaam, Tanzania
| Autor: | Chunfu Yang, Roland O Swai, Charles Kagoma, Tabitha Kibuka, Geofrey R Somi, Karidja Diallo, Eligius Lyamuya, Tulli Tuhuma, Sidibe Kassim, Diane E Bennett |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
medicine.medical_specialty Genotype National Health Programs DNA Mutational Analysis HIV Infections Drug resistance World Health Organization Tanzania Survey methodology Acquired immunodeficiency syndrome (AIDS) HIV Seroprevalence Pregnancy Internal medicine Drug Resistance Viral Medicine Humans Pharmacology (medical) Sida Genotyping Pharmacology biology business.industry Reverse Transcriptase Polymerase Chain Reaction Prenatal Care biology.organism_classification medicine.disease Virology Infectious Diseases Treatment Outcome Anti-Retroviral Agents Mutation HIV-1 Female Viral disease business Sentinel Surveillance HIV drug resistance |
| Zdroj: | Antiviral therapy. 13 |
| ISSN: | 1359-6535 |
| Popis: | BackgroundIn resource-limited settings where antiretroviral treatment (ART) access is being scaled-up, the World Health Organization (WHO) recommends surveillance of transmitted HIV drug resistance (HIVDR). We used the WHO HIVDR threshold survey method to assess transmitted HIVDR in Dar es Salaam where ART was introduced in 1995 and where ∼11,000 people are currently on ART.MethodsFrom November 2005 to February 2006, dried blood spot (DBS) specimens were made from remnant specimens collected during the national HIV serosurvey from 60 primagravidas ResultsAmong the 60 eligible specimens collected, 50 DBS were successfully amplified using RT-PCR. Sequencing was performed on the first 39 specimens: 13 (33.3%) were subtype A1, 13 (33.3%) subtype C, and 4 (10.3%) subtype D, the remainder differed in the closest subtype based on protease versus reverse transcriptase. No resistance mutations were seen; HIVDR to all drug classes was categorized as ConclusionsOur survey indicates that prevalence of transmitted HIVDR among recently infected pregnant women in Dar es Salaam is low ( |
| Databáze: | OpenAIRE |
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