Interlaboratory proficiency processing scheme in CSF aliquoting: implementation and assessment based on biomarkers of Alzheimer’s disease

Autor: Sebastian Brandner, Fay Betsou, Johannes Kornhuber, Sandra Mendoza, Olga Kofanova, Kaj Blennow, Silvia Paciotti, Michael H.A. Roehrl, Piotr Lewczuk, Barbara Mroczko, Dominik Strapagiel, Natalia Ermann, Jonathan Vogelgsang, Amélie Gaignaux, Charlotte E. Teunissen
Přispěvatelé: Amsterdam Neuroscience - Neurodegeneration, Clinical chemistry
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Oncology
Biobanking
medicine.medical_specialty
Laboratory Proficiency Testing
Cognitive Neuroscience
Operating procedures
Coefficient of variation
tau Proteins
lcsh:RC346-429
Central laboratory
lcsh:RC321-571
03 medical and health sciences
0302 clinical medicine
Medizinische Fakultät
Alzheimer Disease
Internal medicine
Albumins
medicine
Disease biomarker
Humans
Alzheimer’s disease
Biomarker
Cerebrospinal fluid
Laboratory standardization
ddc:610
Phosphorylation
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
lcsh:Neurology. Diseases of the nervous system
Amyloid beta-Peptides
business.industry
Geriatrics gerontology
Research
Reproducibility of Results
Total variability
Peptide Fragments
030104 developmental biology
Neurology
Biomarker (medicine)
Neurology (clinical)
business
030217 neurology & neurosurgery
Biomarkers
Zdroj: Alzheimer's Research and Therapy, 10(1):87. BioMed Central
Alzheimer’s Research & Therapy, Vol 10, Iss 1, Pp 1-9 (2018)
Alzheimer's Research & Therapy
Lewczuk, P, Gaignaux, A, Kofanova, O, Ermann, N, Betsou, F, Brandner, S, Mroczko, B, Blennow, K, Strapagiel, D, Paciotti, S, Vogelgsang, J, Roehrl, M H, Mendoza, S, Kornhuber, J & Teunissen, C 2018, ' Interlaboratory proficiency processing scheme in CSF aliquoting: Implementation and assessment based on biomarkers of Alzheimer's disease ', Alzheimer's Research and Therapy, vol. 10, no. 1, 87 . https://doi.org/10.1186/s13195-018-0418-3
ISSN: 1758-9193
DOI: 10.1186/s13195-018-0418-3
Popis: Background In this study, we tested to which extent possible between-center differences in standardized operating procedures (SOPs) for biobanking of cerebrospinal fluid (CSF) samples influence the homogeneity of the resulting aliquots and, consequently, the concentrations of the centrally analyzed selected Alzheimer’s disease biomarkers. Methods Proficiency processing samples (PPSs), prepared by pooling of four individual CSF samples, were sent to 10 participating centers, which were asked to perform aliquoting of the PPSs into two secondary aliquots (SAs) under their local SOPs. The resulting SAs were shipped to the central laboratory, where the concentrations of amyloid beta (Aβ) 1–42, pTau181, and albumin were measured in one run with validated routine analytical methods. Total variability of the concentrations, and its within-center and between-center components, were analyzed with hierarchical regression models. Results We observed neglectable variability in the concentrations of pTau181 and albumin across the centers and the aliquots. In contrast, the variability of the Aβ1–42 concentrations was much larger (overall coefficient of variation 31%), with 28% of the between-laboratory component and 10% of the within-laboratory (i.e., between-aliquot) component. We identified duration of the preparation of the aliquots and the centrifugation force as two potential confounders influencing within-center variability and biomarker concentrations, respectively. Conclusions Proficiency processing schemes provide objective evidence for the most critical preanalytical variables. Standardization of these variables may significantly enhance the quality of the collected biospecimens. Studies utilizing retrospective samples collected under different local SOPs need to consider such differences in the statistical evaluations of the data.
Databáze: OpenAIRE
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