Reproductive barriers in cassava: Factors and implications for genetic improvement
| Autor: | Luciano Rogério Braatz de Andrade, Everton Hilo de Souza, Alfredo Augusto Cunha Alves, Eder Jorge de Oliveira, Massaine Bandeira e Sousa |
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| Rok vydání: | 2021 |
| Předmět: |
Manihot
Fruit and Seed Anatomy Physiology Science Pollen Tube Plant Science Flowers Biology medicine.disease_cause Polymorphism Single Nucleotide Intraspecific competition Ovules Human fertilization Pollen medicine Ovule Pollination Flower Anatomy Cassava Multidisciplinary Plant Anatomy Organisms food and beverages Biology and Life Sciences Eukaryota Reproductive isolation Plants Horticulture Plant Breeding Pistils Stigma Germination Plant Physiology Seeds Medicine Pollen tube Shrubs Hand-pollination Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 11, p e0260576 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Cassava breeding is hampered by high flower abortion rates that prevent efficient recombination among promising clones. To better understand the factors causing flower abortion and propose strategies to overcome them, we 1) analyzed the reproductive barriers to intraspecific crossing, 2) evaluated pollen-pistil interactions to maximize hand pollination efficiency, and 3) identified the population structure of elite parental clones. From 2016 to 2018, the abortion and fertilization rates of 5,748 hand crossings involving 91 parents and 157 progenies were estimated. We used 16,300 single nucleotide polymorphism markers to study the parents’ population structure via discriminant analysis of principal components, and three clusters were identified. To test for male and female effects, we used a mixed model in which the environment (month and year) was fixed, while female and male (nested to female) were random effects. Regardless of the population structure, significant parental effects were identified for abortion and fertilization rates, suggesting the existence of reproductive barriers among certain cassava clones. Matching ability between cassava parents was significant for pollen grains that adhered to the stigma surface, germinated pollen grains, and the number of fertilized ovules. Non-additive genetic effects were important to the inheritance of these traits. Pollen viability and pollen-pistil interactions in cross- and self-pollination were also investigated to characterize pollen-stigma compatibility. Various events related to pollen tube growth dynamics indicated fertilization abnormalities. These abnormalities included the reticulated deposition of callose in the pollen tube, pollen tube growth cessation in a specific region of the stylet, and low pollen grain germination rate. Generally, pollen viability and stigma receptivity varied depending on the clone and flowering stage and were lost during flowering. This study provides novel insights into cassava reproduction that can assist in practical crossing and maximize the recombination of contrasting clones. |
| Databáze: | OpenAIRE |
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