Phosphoenolpyruvate: sugar phosphotransferase system from the hyperthermophilic Thermoanaerobacter tengcongensis
| Autor: | Vera Navdaeva, Ulrich Baumann, Andreas Zurbriggen, Philipp Schneider, Bernhard Erni, Sandro Waltersperger, Andreas Grieder, Priska Bähler, Anselm Erich Oberholzer, Christoph Bächler |
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| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Protein Denaturation Hot Temperature Protein Conformation education Thermoanaerobacter Biology Crystallography X-Ray Biochemistry Models Biological Phosphotransferase chemistry.chemical_compound Enzyme Stability Transferase Cloning Molecular Phosphorylation Phosphoenolpyruvate Sugar Phosphotransferase System chemistry.chemical_classification Active site Fructose PEP group translocation biology.organism_classification Enzyme chemistry cardiovascular system biology.protein Mutant Proteins Phosphoenolpyruvate carboxykinase |
| Zdroj: | Biochemistry. 50(7) |
| ISSN: | 1520-4995 |
| Popis: | Thermoanaerobacter tengcongensis is a thermophilic eubacterium that has a phosphoenolpyruvate (PEP) sugar phosphotransferase system (PTS) of 22 proteins. The general PTS proteins, enzyme I and HPr, and the transporters for N-acetylglucosamine (EIICB(GlcNAc)) and fructose (EIIBC(Fru)) have thermal unfolding transitions at ∼90 °C and a temperature optimum for in vitro sugar phosphotransferase activity of 65 °C. The phosphocysteine of a EIICB(GlcNAc) mutant is unusually stable at room temperature with a t(1/2) of 60 h. The PEP binding C-terminal domain of enzyme I (EIC) forms a metastable covalent adduct with PEP at 65 °C. Crystallization of this adduct afforded the 1.68 Å resolution structure of EIC with a molecule of pyruvate in the active site. We also report the 1.83 Å crystal structure of the EIC-PEP complex. The comparison of the two structures with the apo form and with full-length EI shows differences between the active site side chain conformations of the PEP and pyruvate states but not between the pyruvate and apo states. In the presence of PEP, Arg465 forms a salt bridge with the phosphate moiety while Glu504 forms salt bridges with Arg186 and Arg195 of the N-terminal domain of enzyme I (EIN), which stabilizes a conformation appropriate for the in-line transfer of the phosphoryl moiety from PEP to His191. After transfer, Arg465 swings 4.8 Å away to form an alternative salt bridge with the carboxylate of Glu504. Glu504 loses the grip of Arg186 and Arg195, and the EIN domain can swing away to hand on the phosphoryl group to the phosphoryl carrier protein HPr. |
| Databáze: | OpenAIRE |
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