Marek’s disease herpesvirus vaccines integrate into chicken host chromosomes yet lack a virus-host phenotype associated with oncogenic transformation
Autor: | Mary E. Delany, Hans H. Cheng, Marla C. McPherson |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
040301 veterinary sciences Marek Disease Vaccines Virus Integration Biology Serogroup Marek’s disease virus Genome Chromosomes Virus Viral integration 0403 veterinary science Cytogenetics 03 medical and health sciences Immunity Immunology and Microbiology(all) Marek Disease Animals Alphaherpesvirus Herpesvirus 2 Gallid Poultry Diseases Genetics General Veterinary General Immunology and Microbiology Viral Vaccine Public Health Environmental and Occupational Health Viral Vaccines 04 agricultural and veterinary sciences Cell Transformation Viral Chicken Virology veterinary(all) Vaccination Phenotype 030104 developmental biology Infectious Diseases Viral replication Molecular Medicine Oncogenic Viruses Chickens Vaccine Oncovirus |
Zdroj: | Vaccine. 34(46):5554-5561 |
ISSN: | 0264-410X |
DOI: | 10.1016/j.vaccine.2016.09.051 |
Popis: | Marek’s disease (MD) is a lymphotropic and oncogenic disease of chickens that can lead to death in susceptible and unvaccinated host birds. The causative pathogen, MD virus (MDV), a highly oncogenic alphaherpesvirus, integrates into host genome near the telomeres. MD occurrence is controlled across the globe by biosecurity, selective breeding for enhanced MD genetic resistance, and widespread vaccination of flocks using attenuated serotype 1 MDV or other serotypes. Despite over 40 years of usage, the specific mechanism(s) of MD vaccine-related immunity and anti-tumor effects are not known. Here we investigated the cytogenetic interactions of commonly used MD vaccine strains of all three serotypes (HVT, SB-1, and Rispens) with the host to determine if all were equally capable of host genome integration. We also studied the dynamic profiles of chromosomal association and integration of the three vaccine strains, a first for MD vaccine research. Our cytogenetic data provide evidence that all three MD vaccine strains tested integrate in the chicken host genome as early as 1 day after vaccination similar to oncogenic strains. However, a specific, transformation-associated virus-host phenotype observed for oncogenic viruses is not established. Our results collectively provide an updated model of MD vaccine-host genome interaction and an improved understanding of the possible mechanisms of vaccinal immunity. Physical integration of the oncogenic MDV genome into host chromosomes along with cessation of viral replication appears to have joint signification in MDV’s ability to induce oncogenic transformation. Whereas for MD vaccine serotypes, a sustained viral replication stage and lack of the chromosome-integrated only stage were shared traits during early infection. |
Databáze: | OpenAIRE |
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