Accurate whole genome sequencing as the ultimate genetic test
| Autor: | George M. Church, Brock A. Peters, Xun Xu, Radoje Drmanac, Clifford Reid |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Sanger sequencing
Genetics Whole genome sequencing Massive parallel sequencing Genome Human Biochemistry (medical) Clinical Biochemistry Sequence Analysis DNA Biology DNA sequencing symbols.namesake Sequencing by hybridization symbols Humans Human genome Genetic Testing DNA microarray Personal genomics Genome-Wide Association Study |
| Zdroj: | Clinical chemistry. 61(1) |
| ISSN: | 1530-8561 |
| Popis: | Featured Article: Drmanac R, Sparks AB, Callow MJ, Halpern AL, Burns NL, Kermani BG, et al. Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays. Science 2010;327:78–81.4 Even 30 years ago, it was obvious that Sanger sequencing had limited throughput, and a more efficient process could replace many tedious gene and genome mapping projects. It would take until the mid-2000s for massively parallel sequencing (MPS)5 technologies to demonstrate they could overtake the Sanger sequencing hegemony. Our paper was not the first description of a viable MPS technology, but it firmly established that human whole genome sequencing (WGS) could be done affordably ( |
| Databáze: | OpenAIRE |
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