Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid

Autor: Nikolai Verlov, Roman Kamyshinsky, Gaspar Gavrilov, Tatiana Shtam, Anastasia Kudrevatykh, A. Emelyanov, Luiza Garaeva, S.N. Pchelina, Andrey L. Konevega, Irina Miliukhina, Yulia Zabrodskaya
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Cryo-electron microscopy
Physiology
Cell Membranes
Lipid Bilayers
Centrifugation
Exosomes
Nervous System
0302 clinical medicine
Cerebrospinal fluid
Spectrum Analysis Techniques
Medicine and Health Sciences
Electron Microscopy
Lipid bilayer
Cerebrospinal Fluid
Microscopy
Multidisciplinary
Movement Disorders
medicine.diagnostic_test
Chemistry
Vesicle
Neurodegenerative Diseases
Parkinson Disease
Middle Aged
Flow Cytometry
Cell biology
Body Fluids
Separation Processes
Neurology
Spectrophotometry
030220 oncology & carcinogenesis
Medicine
Female
Cytophotometry
Cellular Structures and Organelles
Anatomy
Research Article
Cell signaling
Science
Nanoparticle tracking analysis
Research and Analysis Methods
Tetraspanin 29
Flow cytometry
03 medical and health sciences
Extracellular Vesicles
medicine
Animals
Humans
Vesicles
Aged
Cryoelectron Microscopy
Biology and Life Sciences
Electron Cryo-Microscopy
Cell Biology
Microvesicles
MicroRNAs
030104 developmental biology
Nanoparticles
Ultracentrifugation
Biomarkers
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 1, p e0227949 (2020)
ISSN: 1932-6203
Popis: Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for cell communication and physiology. EVs are found in many human biological fluids, including blood, breast milk, urine, cerebrospinal fluid (CSF), ejaculate, saliva etc. These nano-sized vesicles contain proteins, mRNAs, microRNAs, non-coding RNAs and lipids that are derived from producing cells. EVs deliver complex sets of biological information to recipient cells thereby modulating their behaviors by their molecular cargo. In this way EVs are involved in the pathological development and progression of many human disorders, including neurodegenerative diseases. In this study EVs purified by ultracentrifugation from CSF of patients with Parkinson's disease (PD) and individuals of the comparison group were characterized using nanoparticle tracking analysis, flow cytometry and cryo-electron microscopy. Vesicular size and the presence of exosomal marker CD9 on the surface provided evidence that most of the EVs were exosome-like vesicles. Cryo-electron microscopy allowed us to visualize a large spectrum of extracellular vesicles of various size and morphology with lipid bilayers and vesicular internal structures. Thus, we described the diversity and new characteristics of the vesicles from CSF suggesting that subpopulations of EVs with different and specific functions may exist.
Databáze: OpenAIRE
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