Identification of Rubisco anxiolytic-like peptides (rALPs) by comprehensive analysis of spinach green leaf protein digest
| Autor: | Saeko Kimura, Hideyuki Suzuki, Ryuhei Kanamoto, Masaru Sato, Kousaku Ohinata, Atsushi Kurabayashi, Mayumi Hosokawa, Junya Nakato, Yuki Tokuyama, Tomoki Uchida |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.drug_class Ribulose-Bisphosphate Carboxylase Biophysics Administration Oral Mice Inbred Strains Peptide Anxiety Photosynthesis Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Spinacia oleracea Oral administration medicine Animals Molecular Biology Cells Cultured Plant Proteins chemistry.chemical_classification biology RuBisCO Carbon fixation Cell Biology Receptor antagonist biology.organism_classification Plant Leaves 030104 developmental biology Enzyme Anti-Anxiety Agents chemistry biology.protein Spinach Peptides 030217 neurology & neurosurgery |
| Zdroj: | Biochemical and Biophysical Research Communications. 505:1050-1056 |
| ISSN: | 0006-291X |
| Popis: | Rubisco, an enzyme for photosynthetic carbon dioxide fixation, is a major green leaf protein and known as the most abundant protein on the Earth. We found that Rubisco digested mimicking gastrointestinal enzymatic conditions exhibited anxiolytic-like effects after oral administration in mice. Based on a comprehensive peptide analysis of the digest using nanoLC-Orbitrap-MS and the structure-activity relationship of known anxiolytic-like peptides, we identified SYLPPLTT, SYLPPLT and YHIEPV [termed Rubisco anxiolytic-like peptide (rALP)-1, rALP-1(1–7) and rALP-2, respectively], which exhibited potent anxiolytic-like effects after oral administration. The anxiolytic-like effects of rALP-1/rALP-1(1–7) were blocked by a serotonin 5-HT1A receptor antagonist, whereas rALP-2-induced effects were inhibited by a δ-opioid receptor antagonist. In conclusion, novel Rubisco-derived anxiolytic-like peptides, rALP-1/rALP-1(1–7) and rALP-2, act via independent neural pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect