Mesenchymal stem cells from a hypoxic culture improve nerve regeneration
| Autor: | Ting-Chen Tseng, Yu-Ting Liao, Shih-Chieh Hung, Jung-Pan Wang, Shan-hui Hsu, Szu-Hsien Wu, En-Rung Chiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Senescence
Myelinated nerve fiber 0206 medical engineering Biomedical Engineering Medicine (miscellaneous) 02 engineering and technology Mesenchymal Stem Cell Transplantation Rats Sprague-Dawley Biomaterials 03 medical and health sciences Gastrocnemius muscle stomatognathic system In vivo medicine Animals Humans Cell Shape Cells Cultured 030304 developmental biology Neurons 0303 health sciences Chemistry Cartilage Cell Membrane Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Hypoxia (medical) Sciatic Nerve 020601 biomedical engineering Cell Hypoxia In vitro Nerve Regeneration Cell biology Disease Models Animal medicine.anatomical_structure Gene Expression Regulation medicine.symptom |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 14:1804-1814 |
| ISSN: | 1932-7005 1932-6254 |
| DOI: | 10.1002/term.3136 |
| Popis: | Repairing the peripheral nerves following a segmental defect injury remains surgically challenging. Because of some disadvantages of nerve grafts, nerve regeneration, such as conduits combined with bone marrow-derived mesenchymal stem cells (BMSCs), may serve as an alternative. BMSCs expand under hypoxic conditions, decrease in senescence, and increase in proliferation and differentiation potential into the bone, fat, and cartilage. The purpose of this study was to investigate whether BMSCs increased the neuronal differentiation potential following expansion under hypoxic conditions. Isolated human BMSCs (hBMSCs) expand under hypoxia or normoxia, and neuronal differentiation proceeds under normoxia. in vitro tests revealed hypoxia culture enhanced the RNA and protein expression of neuronal markers. The electrophysiology of hBMSC-differentiated neuron-like cells was also enhanced by the hypoxia culturing. Our animal model indicated that the potential treatment of hypoxic rat BMSCs (rBMSCs) was better than that of normoxic rBMSCs because the conduit with the hypoxic rBMSCs injection demonstrated the highest recovery rate of gastrocnemius muscle weights. There were more toluidine blue-stained myelinated nerve fibers in the hypoxic rBMSCs group than in the normoxic group. To sum up, BMSCs cultured under hypoxia increased the potential of neuronal differentiation both in vivo and in vitro. |
| Databáze: | OpenAIRE |
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