Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation
| Autor: | Leila Haery, Benjamin E. Deverman, Katherine S. Matho, Ali Cetin, Kenton Woodard, Connie Cepko, Karen I. Guerin, Meghan A. Rego, Ina Ersing, Susanna M. Bachle, Joanne Kamens, Melina Fan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
viral vectors Computer science Neuroscience (miscellaneous) Neuronal targeting virus technologies Computational biology Review Gene delivery medicine.disease_cause lcsh:RC321-571 lcsh:QM1-695 Viral vector 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine gene delivery lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Adeno-associated virus Cell specific cell-type specificity AAV lcsh:Human anatomy 030104 developmental biology intersectional methods targeted neuronal manipulation Anatomy 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Neuroanatomy Frontiers in Neuroanatomy, Vol 13 (2019) |
| ISSN: | 1662-5129 |
| Popis: | Cell-type-specific expression of molecular tools and sensors is critical to construct circuit diagrams and to investigate the activity and function of neurons within the nervous system. Strategies for targeted manipulation include combinations of classical genetic tools such as Cre/loxP and Flp/FRT, use of cis-regulatory elements, targeted knock-in transgenic mice, and gene delivery by AAV and other viral vectors. The combination of these complex technologies with the goal of precise neuronal targeting is a challenge in the lab. This report will discuss the theoretical and practical aspects of combining current technologies and establish best practices for achieving targeted manipulation of specific cell types. Novel applications and tools, as well as areas for development, will be envisioned and discussed. |
| Databáze: | OpenAIRE |
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