Hypotensive effect of aqueous extract of Averrhoa carambola L. (Oxalidaceae) in rats: an in vivo and in vitro approach
| Autor: | Geraldo Alves-da-Silva, Roseli Soncini, Alexandre Giusti-Paiva, Lidiane Orlandi, Gabriel de Oliveira Isac Moraes, Valdemar A. Paffaro, Marcelo Henrique dos Santos, Antonio C. Bento, Michael Brian Santiago, André Luiz M. Peloso |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Mean arterial pressure Muscle Relaxation Aorta Thoracic Blood Pressure Pharmacology In Vitro Techniques Averrhoa carambola Muscle Smooth Vascular Phenylephrine In vivo medicine.artery Drug Discovery medicine Extracellular Animals Rats Wistar Antihypertensive Agents Aorta Oxalidaceae Plants Medicinal biology Traditional medicine business.industry Plant Extracts Water biology.organism_classification Calcium Channel Blockers In vitro Rats Plant Leaves Ethnopharmacology Hypertension Medicine Traditional business Brazil medicine.drug Phytotherapy |
| Zdroj: | Journal of ethnopharmacology. 133(2) |
| ISSN: | 1872-7573 |
| Popis: | Aim of the study Averrhoa carambola L. (Oxalidaceae) leaves are used in Brazilian traditional medicine to treat hypertension. This study was conducted to evaluate the hypotensive effect of the aqueous extract of Averrhoa carambola (AEAc) and its underlying mechanisms in the isolated rat aorta. Materials and methods The effect of AEAc on the mean arterial pressure (MAP) was determined in vivo in anesthetized rats. In vitro , thoracic aortic rings were isolated and suspended in organ baths, and the effects of AEAc were studied by means of isometric tension recording experiments. In HPLC analysis, the fingerprint chromatogram of AEAc was established. Results In normotensive rats, AEAc (12.5–50.0 mg/kg, i.v.) induced dose-dependent hypotension. In vitro , AEAc caused a depression in the E max response to phenylephrine without a change in sensibility. Also, in a depolarized Ca 2+ -free medium, AEAc inhibited CaCl 2 -induced contractions and caused a concentration-dependent rightward shift of the response curves, indicating that AEAc inhibited the contractile mechanisms involving extracellular Ca 2+ influx. Conclusions These results demonstrate the hypotensive effects of AEAc, and these effects may, in part, be due to the inhibition of Ca 2+ , which supports previous claims of its traditional use. |
| Databáze: | OpenAIRE |
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