Reduction of E. coli adherence to rubber slices treated with phospholipids
| Autor: | Torkel Wadström, Bo G. Persson, Li-Qing Wang, Åsa Ljungh, Jian-Lin Yu, Erzsébet Jakab, Stig Bengmark, Roland Andersson |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Microbiology (medical) Time Factors Phospholipid Phosphatidylinositols medicine.disease_cause Bacterial Adhesion Pathology and Forensic Medicine Rats Sprague-Dawley chemistry.chemical_compound Natural rubber In vivo Escherichia coli medicine Animals Immunology and Allergy Phosphatidylinositol Phospholipids Chromatography biology Temperature General Medicine Adhesion biology.organism_classification Enterobacteriaceae In vitro Fibronectins Rats Biochemistry chemistry visual_art Phosphatidylcholines visual_art.visual_art_medium Rubber |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1600-0463 0903-4641 |
| DOI: | 10.1111/j.1699-0463.1993.tb00150.x |
| Popis: | The present study aimed at modifying the surface of biliary drain material to reduce bacterial adherence. The adherence of cells of seven E. coli strains to rubber slices treated with phosphatidylcholin (PC) or phosphatidylinositol (PI) and the adherence of cells of E. coli strain NG7C to PC- or PI-treated rubber slices implanted in the common bile duct in rats were studied in vitro. The rubber slices were incubated with 1 x 10(7) cfu radiolabeled E. coli cells/ml at 37 degrees C for 60 min and then drained and washed thrice in 2 ml PBS, and adherent E. coli cells were quantified by radioactivity counting. The results show that both PC and PI absorbed on the surface of slices reduced the adherence of E. coli cells in at least two ways, i.e. by changing surface properties in vitro and by reducing deposition of host-derived molecules on phospholipid-treated surfaces in vivo. The results may be of use for modification of the biomaterial surface in the clinical situation. |
| Databáze: | OpenAIRE |
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