The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila
| Autor: | Kiichiro Taniguchi, Ryunosuke Minami, Akihiko Kokuryo, Takashi Adachi-Yamada, Takao Imano, Seiko Sugimori, Hideki Nakagoshi, Naoko Watanabe, Miyuki Wakabayashi |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Exocrine gland Anatomy and Physiology Gene Expression lcsh:Medicine Sexual Behavior Animal Seminal vesicle Drosophila Proteins lcsh:Science Multidisciplinary Proventriculus Animal Models Fecundity Immunohistochemistry Cell biology medicine.anatomical_structure Intercellular Signaling Peptides and Proteins Drosophila Female RNA Interference Drosophila Protein Research Article medicine.medical_specialty Genetic Vectors Genitalia Male Biology Molecular Genetics Model Organisms Exocrine Glands Semen Internal medicine Genetics medicine Animals Homeodomain Proteins Analysis of Variance Gene Expression Profiling lcsh:R Reproductive System Molecular Development Sperm Male accessory gland Fertility Endocrinology Homeobox lcsh:Q Peptides Developmental Biology Transcription Factors |
| Zdroj: | PLoS ONE, Vol 7, Iss 3, p e32302 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity. |
| Databáze: | OpenAIRE |
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