Investigation of olive mill wastewater (OMW) ozonation efficiency with the use of a battery of selected ecotoxicity and human toxicity assays
| Autor: | Maria-Sophia Vidali, Theodoros T. Vgenis, Margarita Andreas Dareioti, Michael Kornaros, Ioanna Efthimiou, Dimitris Vlastos, Sofia Siorou, Stefanos Dailianis |
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| Rok vydání: | 2015 |
| Předmět: |
Hemocytes
Health Toxicology and Mutagenesis Industrial Waste Wastewater Aquatic Science medicine.disease_cause Lipid peroxidation chemistry.chemical_compound Ozone Phenols Olea Toxicity Tests medicine Animals Humans Bioassay Lymphocytes Effluent Acute toxicity Bivalvia chemistry Environmental chemistry Toxicity Anostraca Ecotoxicity Oxidation-Reduction Water Pollutants Chemical Genotoxicity |
| Zdroj: | Aquatic Toxicology. 164:135-144 |
| ISSN: | 0166-445X |
| Popis: | The effects of olive mill wastewater (OMW) on a battery of biological assays, before and during the ozonation process, were investigated in order to assess ozone’s efficiency in removing phenolic compounds from OMW and decreasing the concomitant OMW toxicity. Specifically, ozonated-OMW held for 0, 60, 120, 300, 420, 540 min in a glass bubble reactor, showed a drastic reduction of OMW total phenols (almost 50%) after 300 min of ozonation with a concomitant decrease of OMW toxicity. In particular, the acute toxicity test primarily performed in the fairy shrimp Thamnocephalus platyurus (Thamnotoxkit F™ screening toxicity test) showed a significant attenuation of OMW-induced toxic effects, after ozonation for a period of 120 and in a lesser extent 300 min, while further treatment resulted in a significant enhancement of ozonated-OMW toxic effects. Furthermore, ozonated-OMW-treated mussel hemocytes showed a significant attenuation of the ability of OMW to cause cytotoxic (obtained by the use of NRRT assay) effects already after an ozonation period of 120 and to a lesser extent 300 min. In accordance with the latter, OMW-mediated oxidative (enhanced levels of superoxide anions and lipid peroxidation by-products) and genotoxic (induction of DNA damage) effects were diminished after OMW ozonation for the aforementioned periods of time. The latter was also revealed by the use of cytokinesis block micronucleus (CBMN) assay in human lymphocytes exposed to different concentrations of both raw- and ozonated-OMW for 60, 120 and 300 min. Those findings revealed for a first time the existence of a critical time point during the OMW ozonation process that could be fundamentally used for evaluating OMW ozonation as a pretreatment method of OMW. |
| Databáze: | OpenAIRE |
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