Mitochondrial DNA in Fresh versus Frozen Embryo Culture Media of Polycystic Ovarian Syndrome Patients Undergoing Invitro Fertilization: A Possible Predictive Marker of a Successful Pregnancy
| Autor: | Hussein Al-Sawaf, Ghadir A. Sayed, Ahmed H. Al-Sawaf, Iman Hassan Ibrahim, Ahmed M. Maged, Mohamed Saeid |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Andrology 03 medical and health sciences 0302 clinical medicine Pharmacogenomics and Personalized Medicine Embryo cryopreservation frozen embryos transfer Medicine fresh embryos transfer Original Research Pharmacology Predictive marker mtDNA business.industry Embryo culture Embryo Polycystic ovary female genital diseases and pregnancy complications Embryo transfer Pregnancy rate 030104 developmental biology polycystic ovary syndrome Genetic marker 030220 oncology & carcinogenesis Molecular Medicine business |
| Zdroj: | Pharmacogenomics and Personalized Medicine |
| ISSN: | 1178-7066 |
| DOI: | 10.2147/pgpm.s284064 |
| Popis: | Purpose Frozen embryos transfer (ET) may improve the live-birth and reduce rates of ovarian hyperstimulation in polycystic ovary syndrome (PCOS) patients. Morphological criteria are the classical way for embryo selection, yet recently, many biochemical and genetic markers have been developed. This study aimed to compare fresh and frozen ET using the mtDNA/gDNA ratio of embryo secretome and the possibility of using this ratio as a predictive marker of PCOS pregnancy rate. Subjects and Methods One hundred PCOS patients undergoing IVF were chosen according to Rotterdam criteria and divided into two groups. Group I (50 with fresh ET), group II (50 with frozen ET), and otherwise 33 apparently healthy women as a control group with fresh ET. We then carried out absolute quantification of embryo culture media mtDNA and gDNA by real-time PCR. Results mtDNA/gDNA ratio was significantly low in PCOS embryo culture media in comparison with control. Additionally, while the mtDNA/gDNA ratio was significantly high in pregnant PCOS embryo culture media, it was high, though not statistically significant, in the fresh ET than frozen ET group. mtDNA/gDNA ratio sensitivity and specificity in PCOS embryo culture media as a predictive value of pregnancy rate were (86% and 96%, respectively). Conclusion mtDNA/gDNA ratio measurement in PCOS embryo culture media is a novel marker that can be clinically applied as a predictive value of the quality of the morphologically good embryo. Video abstract Point your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: https://youtu.be/uqKkQgRrql4 |
| Databáze: | OpenAIRE |
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