Importance of parental genome balance in the generation of novel yet heritable epigenetic and transcriptional states during doubled haploid breeding
| Autor: | Guy C. Barker, Javier Antunez-Sanchez, José F. Gutierrez-Marcos, Claude Becker, Jonathan Price, Anjar Wibowo, Nosheen Hussain, Graham R. Teakle, Ranjith K Papareddy, Detlef Weigel |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences fungi Biology biology.organism_classification 01 natural sciences Genome Phenotype 03 medical and health sciences Doubled haploidy Transcriptional regulation Brassica oleracea Epigenetics Plant breeding Ploidy 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/812347 |
| Popis: | BackgroundDoubling the genome contribution of haploid plants has accelerated breeding in most cultivated crop species. Although plant doubled haploids are isogenic in nature, they frequently display unpredictable phenotypes, thus limiting the potential of this technology. Therefore, being able to predict the factors implicated in this phenotypic variability could accelerate the generation of desirable genomic combinations and ultimately plant breeding.ResultsWe use computational analysis to assess the transcriptional and epigenetic dynamics taking place during doubled haploids generation in the genome ofBrassica oleracea. We observe that doubled haploid lines display unexpected levels of transcriptional and epigenetic variation, and that this variation is largely due to imbalanced contribution of parental genomes. We reveal that epigenetic modification of transposon-related sequences during DH breeding contributes to the generation of unpredictable yet heritable transcriptional states. Targeted epigenetic manipulation of these elements using dCas9-hsTET3 confirms their role in transcriptional regulation. We have uncovered a hitherto unknown role for parental genome balance in the transcriptional and epigenetic stability of doubled haploids.ConclusionsThis is the first study that demonstrates the importance of parental genome balance in the transcriptional and epigenetic stability of doubled haploids, thus enabling predictive models to improve doubled haploid-assisted plant breeding. |
| Databáze: | OpenAIRE |
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