Nine viruses from eight lineages exhibiting new evolutionary modes that co-infect a hypovirulent phytopathogenic fungus

Autor: Shufen Cheng, Yanping Fu, Fan Mu, Jichun Jia, Jiatao Xie, Jiasen Cheng, Bo Li, Tao Chen, Daohong Jiang, Yang Lin
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Genome
Biochemistry
Fungal Evolution
Biology (General)
Phylogeny
Genomic organization
Data Management
Viral Genomics
Sclerotinia sclerotiorum
Phylogenetic Analysis
Genomics
Biological Evolution
Enzymes
Phylogenetics
Viral evolution
Horizontal gene transfer
Viruses
Helicases
RNA
Viral
Research Article
Computer and Information Sciences
QH301-705.5
Gene prediction
Immunology
Mycology
Microbial Genomics
Genome
Viral
Biology
Fungal Viruses
Microbiology
Virus
Viral Evolution
Ascomycota
Virology
Genetics
Fungal Genetics
Evolutionary Systematics
Cell Lineage
Gene Prediction
Molecular Biology
Fungal Genomics
Taxonomy
Plant Diseases
Evolutionary Biology
Brassica napus
Organisms
Biology and Life Sciences
Computational Biology
Proteins
RC581-607
biology.organism_classification
Genome Analysis
Organismal Evolution
Plant Leaves
Evolutionary biology
Microbial Evolution
Mycovirus
Enzymology
Parasitology
Immunologic diseases. Allergy
Zdroj: PLoS Pathogens
PLoS Pathogens, Vol 17, Iss 8, p e1009823 (2021)
ISSN: 1553-7374
1553-7366
Popis: Mycoviruses are an important component of the virosphere, but our current knowledge of their genome organization diversity and evolution remains rudimentary. In this study, the mycovirus composition in a hypovirulent strain of Sclerotinia sclerotiorum was molecularly characterized. Nine mycoviruses were identified and assigned into eight potential families. Of them, six were close relatives of known mycoviruses, while the other three had unique genome organizations and evolutionary positions. A deltaflexivirus with a tripartite genome has evolved via arrangement and horizontal gene transfer events, which could be an evolutionary connection from unsegmented to segmented RNA viruses. Two mycoviruses had acquired a second helicase gene by two different evolutionary mechanisms. A rhabdovirus representing an independent viral evolutionary branch was the first to be confirmed to occur naturally in fungi. The major hypovirulence-associated factor, an endornavirus, was finally corroborated. Our study expands the diversity of mycoviruses and potential virocontrol agents, and also provides new insights into virus evolutionary modes including virus genome segmentation.
Author summary Identification of mycoviruses in phytopathogenic fungi is necessary for understanding the origin of viruses and developing virocontrol strategies to protect plants. Nine mycoviruses with RNA genomes were identified in a hypovirulent strain of Sclerotinia sclerotiorum and were classified into eight potential viral families, suggesting that the composition of mycoviral communities was complex in this single fungal strain. They included four previously characterized mycoviruses and three distant relatives of known mycoviruses, as well as the first reports of a deltaflexivirus with a tripartite genome, and a fungal rhabdovirus. In addition, we found an endornavirus associated with hypovirulence in a phytopathogenic fungus. Our study makes a significant contribution because it not only expands the diversity-related knowledge of mycoviruses and potential virocontrol agents, but also provides new insights into mycovirus evolution.
Databáze: OpenAIRE
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