Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms
Autor: | Joanna Frąckiewicz, Bogdan Dębski, Agata Wawrzyniak, Magdalena Górnicka, Małgorzata Drywień |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine medicine.medical_specialty DNA damage Thiobarbituric acid alpha-Tocopherol Medicine (miscellaneous) Physical exercise medicine.disease_cause Antioxidants General Biochemistry Genetics and Molecular Biology Lipid peroxidation Random Allocation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endurance training Physical Conditioning Animal Internal medicine Internal Medicine medicine TBARS Animals Pharmacology (medical) Rats Wistar Genetics (clinical) business.industry food and beverages 030229 sport sciences Rats Oxidative Stress 030104 developmental biology Endocrinology chemistry Reviews and References (medical) Hepatocytes lipids (amino acids peptides and proteins) Lipid Peroxidation business Oxidative stress |
Zdroj: | Advances in Clinical and Experimental Medicine. 25:673-679 |
ISSN: | 1899-5276 |
Popis: | BACKGROUND Training-induced oxidative stress can be reduced by α-tocopherol. Adequate intake of α-tocopherol could have health benefits for previously untrained young subjects. OBJECTIVES The aim of this study was to determine the effects of training and different doses of α-tocopherol on exercise-induced oxidative stress in rat livers. MATERIAL AND METHODS Young male Wistar rats (n = 40) were randomly divided into eight groups (undergoing training and not undergoing training, given orally administered α-tocopherol doses of 0, 0.5, 1.0 and 4.0 mg). Every day for 10 consecutive days, the rats in the training groups ran for 15 min on a treadmill at 20 m/min to induce oxidative stress. Hepatic oxidative stress was evaluated based on the liver concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and thiobarbituric acid reactive substances (TBARS). RESULTS The liver concentrations of α-tocopherol were significantly influenced by α-tocopherol doses (p < 0.001) and physical exercise (p < 0.001). The liver concentrations of α-tocopherol increased in response to the highest dose (4 mg/d) of α-tocopherol in the non-training groups. In the training groups, the liver concentrations of α-tocopherol were independent of the dose. The levels of TBARS, a marker of lipid peroxidation, were lowest in the training and non-training rats administered 4.0 mg of α-tocopherol. Physical exercise and α-tocopherol doses significantly influenced TBARS concentrations (p = 0.004, p < 0.05). CONCLUSIONS The results of this study indicate that running training causes lipids peroxidation and reduces α-tocopherol levels in the liver, but it does not contribute to DNA damage. Increased liver concentrations of α-tocopherol were found to exert a protective effect against oxidative damage induced by endurance training. An adequate intake of α-tocopherol is important for previously untrained young subjects. |
Databáze: | OpenAIRE |
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