Bipolar, anastral spindle development in artificially activated sea urchin eggs
| Autor: | Calvin Simerly, Mary K. McClellan, Jason Ader, Jessica E. Davis, John H. Henson, Christopher A. Fried, Rudolf Oldenbourg |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Centriole Spindle Apparatus Spindle pole body Article Nuclear Matrix-Associated Proteins Ammonia biology.animal Lytechinus Botany Animals Sea urchin Mitosis Metaphase biology Cell Polarity Antigens Nuclear biology.organism_classification Cell biology Spindle apparatus Centrosome Fertilization embryonic structures Oocytes Female Developmental Biology |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 237(5) |
| ISSN: | 1058-8388 |
| Popis: | The mitotic apparatus of the early sea urchin embryo is the archetype example of a centrosome-dominated, large aster spindle organized by means of the centriole of the fertilizing sperm. In this study, we tested the hypothesis that artificially activated sea urchin eggs possess the capacity to assemble the anastral, bipolar spindles present in many acentrosomal systems. Control fertilized Lytechinus pictus embryos and ammonia-activated eggs were immunolabeled for tubulin, centrosomal material, the spindle pole structuring protein NuMA and the mitotic kinesins MKLP1/Kinesin-6, Eg5/Kinesin-5, and KinI/Kinesin-13. Confocal imaging showed that a subset of ammonia-activated eggs contained bipolar "mini-spindles" that were anastral; displayed metaphase and anaphase-like stages; labeled for centrosomal material, NuMA, and the three mitotic kinesins; and were observed in living eggs using polarization optics. These results suggest that spindle structural and motor proteins have the ability to organize bipolar, anastral spindles in sea urchin eggs activated in the absence of the paternal centriole. |
| Databáze: | OpenAIRE |
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