Experimental varicocoele in rats affects mechanisms that control expression and function of the androgen receptor
| Autor: | Soares, Thiago da Silva [UNIFESP], Fernandes, Sheilla Alessandra Ferreira [UNIFESP], De Lima-Stein, Mariana Leão [UNIFESP], Stumpp, Taiza [UNIFESP], Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP], Lazari, Maria de Fatima Magalhaes [UNIFESP], Porto, Catarina Segreti [UNIFESP], Soares, T. S., Fernandes, S. A. F., Lima, M. L., Lazari, M. F. M., Porto, C. S. |
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| Přispěvatelé: | Dept Pharmacol, Dept Morphol & Genet, Universidade Federal de São Paulo (UNIFESP) |
| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Male medicine.medical_specialty Urology Endocrinology Diabetes and Metabolism Receptors Cell Surface Biology Receptors for Activated C Kinase Receptor for activated C kinase 1 Receptors G-Protein-Coupled chemistry.chemical_compound Endocrinology Downregulation and upregulation GTP-Binding Proteins Internal medicine Testis Varicocele medicine Varicocoele Animals Estrogen Receptor beta Testosterone RNA Messenger Phosphorylation Rats Wistar Receptor Infertility Male Messenger RNA Sertoli Cells Estradiol Estrogen Receptor alpha Tyrosine phosphorylation Spermatozoa Neoplasm Proteins Rats Androgen receptor Reproductive Medicine chemistry Receptors Estrogen Receptors Androgen Oestrogen receptors Estrogen receptor alpha GPER |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| ISSN: | 2047-2927 |
| Popis: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Varicocoele is an important cause of male infertility. Normal male reproductive function and fertility depends on a delicate balance between androgen receptor (AR) and the classic oestrogen receptors ESR1 (ER alpha) and ESR2 (ER beta). Using a model of surgically induced varicocoele in rats, this study aimed to investigate the effects of varicocoele on the expression of AR, ESR1, ESR2 and G-protein coupled oestrogen receptor (GPER). Varicocoele did not affect the mRNA and protein expression of ESR1 and ESR2 in both testes. Varicocoele did not affect the mRNA and protein expression of GPER in the right testis, but slightly reduced the mRNA and increased the protein levels in the left testis. Varicocoele did not affect the mRNA for AR, but reduced the protein levels in both testes. A proteomic approach was used in an attempt to find differentially expressed targets with possible correlation with AR downregulation. Varicocoele caused the differential expression of 29 proteins. Six proteins were upregulated, including the receptor for activated C kinase 1 (RACK1), and 23 were downregulated, including dihydrolipoamide dehydrogenase, alpha-enolase and pyrophosphatase 1. Western blot analysis confirmed that varicocoele upregulated the expression of RACK1, a protein involved with tyrosine phosphorylation and regulation of AR transcriptional activity, AR metabolism and dynamics of the blood-testis barrier. in conclusion, this study suggests that varicocoele affects mechanisms that control AR expression and function. This regulation of AR may play an important role in the varicocoele-induced testicular dysfunction. Furthermore, varicocoele downregulates several other proteins in the testis that may be useful markers of spermatozoa function and male infertility. Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil Dept Morphol & Genet, Dev Biol Lab, São Paulo, Brazil Universidade Federal de São Paulo, Dept Physiol, Escola Paulista Med, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Dept Physiol, Escola Paulista Med, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Dept Physiol, Escola Paulista Med, BR-04044020 São Paulo, Brazil FAPESP: 2007/52471-6 FAPESP: 2010/52306-8 Web of Science |
| Databáze: | OpenAIRE |
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