Inactivation of p16INK4a in Primary Tumors and Cell Lines of Head and Neck Squamous Cell Carcinoma
| Autor: | Jung-Wan Kim, Sun-Young Na, Jin-A Kim, Woon Bok Chung, Yoon-Kyung Sohn, Ho-Seok Kim, Su-Hyung Hong, Hyun-Jung Jang |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Transcription Genetic Biology medicine.disease_cause White People Asian People Tumor Cells Cultured Carcinoma medicine Humans Genes Tumor Suppressor Epigenetics neoplasms Molecular Biology Gene Cyclin-Dependent Kinase Inhibitor p16 Aged Sequence Deletion Mutation Korea Cell Biology General Medicine DNA Methylation Middle Aged medicine.disease Head and neck squamous-cell carcinoma Molecular biology Gene Expression Regulation Neoplastic stomatognathic diseases CpG site Head and Neck Neoplasms Cell culture DNA methylation Carcinoma Squamous Cell Mouth Neoplasms Carrier Proteins Gene Deletion |
| Zdroj: | Molecules and Cells. 10:557-565 |
| ISSN: | 1016-8478 |
| DOI: | 10.1007/s10059-000-0557-8 |
| Popis: | Inactivation of the p16INK4a gene by mutation and deletion is common in head and neck squamous cell carcinoma (HNSCC). The present study demonstrates that hypermethylation of the 5' CpG islands can serve as an alternative mechanism for the inactivation of the p16INK4a gene in this tumor. We studied 11 HNSCC cell lines and 17 oral squamous cell carcinoma (OSCC) primary tumors for p16INK4a gene status by protein/mRNA and DNA genetic/epigenetic analyses to determine the incidence of its inactivation. Our study indicates that: (1) inactivation of p16 protein is frequent in HNSCC cell lines (6/11, 54.5%) and OSCC primary tumors (15/17, 88.2%), (2) inactivation of p16INK4a protein is commonly associated with the presence of gene alteration such as mutation, homozygous deletion and especially aberrant methylation, and (3) genomic sequencing of bisulfite-modified DNA shows that the carcinoma develops a heterogeneous pattern of hypermethylation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink