The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis
Autor: | David J. Brenner, Guy Garty, Mikhail Repin, Sergey Pampou |
---|---|
Rok vydání: | 2019 |
Předmět: |
biodosimetry
Computer science Health Toxicology and Mutagenesis micronucleus assay Blood Donors Centrifugation 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Biodosimetry Image Processing Computer-Assisted Humans Radiology Nuclear Medicine and imaging Letter to the Editor Throughput (business) high-throughput automation 030304 developmental biology 0303 health sciences Centrifuge Radiation Micronucleus Tests Robotics High-Throughput Screening Assays Robotic systems 030220 oncology & carcinogenesis High-content screening Micronucleus test Micronucleus Biotechnology Biomedical engineering |
Zdroj: | Journal of Radiation Research |
Popis: | The cytokinesis-block micronucleus (CBMN) assay is considered to be the most suitable biodosimetry method for automation. Previously, we automated this assay on a commercial robotic biotech high-throughput system (RABiT-II) adopting both a traditional and an accelerated micronucleus protocol, using centrifugation steps for both lymphocyte harvesting and washing, after whole blood culturing. Here we describe further development of our accelerated CBMN assay protocol for use on high-throughput/high content screening (HTS/HCS) robotic systems without a centrifuge. This opens the way for implementation of the CBMN assay on a wider range of commercial automated HTS/HCS systems and thus increases the potential capacity for dose estimates following a mass-casualty radiological event. |
Databáze: | OpenAIRE |
Externí odkaz: |