Effects of anastrozole on Ki-67 antigen expression in the vaginal epithelium of female rats in persistent estrus
| Autor: | Danylo Rafhael Costa-Silva, Mariella de Almeida Melo, Gil Facina, Maria da Conceição Barros-Oliveira, Renato de Oliveira Pereira, Benedito Borges da Silva, Pedro Vitor Lopes-Costa, Diego Cipriano Chagas, Alesse Ribeiro dos Santos, Carine Soares Borges, Jackeline Lopes Viana |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Medicine (General)
Proliferation Anastrozole 030204 cardiovascular system & hematology Epithelium Andrology 03 medical and health sciences Random Allocation 0302 clinical medicine Breast cancer R5-920 Antigen Estrus Medicine Animals 030212 general & internal medicine Aromatase Rats Wistar Persistent estrus biology business.industry Cell growth General Medicine medicine.disease Rats medicine.anatomical_structure Ki-67 Antigen Ki-67 Vagina biology.protein Rat Original Article Female business medicine.drug |
| Zdroj: | Clinics, Vol 75 (2020) Clinics; v. 75 (2020); e1643 Clinics; Vol. 75 (2020); e1643 Clinics Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1980-5322 1807-5932 |
| Popis: | OBJECTIVES: Aromatase inhibitors are the first-choice drugs for the treatment of hormone sensitive breast cancer. However, in addition to the scarcity of studies, there are controversies about their effects on vaginal epithelial cell proliferation in rats, especially those in persistent estrus. METHODS: To investigate vaginal epithelial cell proliferation by Ki-67 antigen expression, persistent estrus was induced in 42 randomly selected rats. These rats were randomly divided into 2 groups: group I (control, n=21), which received 0.1 mL of propylene glycol (vehicle) daily, and group II (experimental, n=21), which received 0.5 mg/kg or 0.125 mg/day of anastrozole diluted with 0.1 mL of propylene glycol. RESULTS: Light microscopy showed a higher concentration of cells with brown Ki-67 stained nuclei in the control compared to the experimental group. The mean percentage of Ki-67 stained nuclei per 500 cells in the vaginal epithelium was 68.64±2.64 and 30.46±2.00 [mean±standard error of the mean (SEM)] in the control and experimental groups, respectively (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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