Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication
| Autor: | Evo Kristina Lindersson Søndergaard, Rikke Bæk, Kim Varming, Malene Jørgensen, Allan Stensballe, Anne Louise Revenfeld |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cell signaling
Vesicle Transwell Biochemistry (medical) Clinical Biochemistry Cell Nanoparticle tracking analysis Cell Communication Contact-independent Extracellular vesicle Biology lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Extracellular Vesicles EV Array Phenotype lcsh:RC254-282 Cell biology medicine.anatomical_structure Membrane Cell culture medicine Original Research Article Primary cell |
| Zdroj: | Revenfeld, A L S, Søndergaard, E K L, Stensballe, A, Bæk, R, Jørgensen, M M & Varming, K 2016, ' Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication ', Journal of Circulating Biomarkers, vol. 5, no. 3, 3 . https://doi.org/10.5772/62580 Journal of Circulating Biomarkers Journal of Circulating Biomarkers, Vol 5, Iss, p 3 (2016) Journal of Circulating Biomarkers, Vol 5 (2016) Volume 5 Issue Godište 2016 Revenfeld, A L S, Søndergaard, E K L, Stensballe, A, Bæk, R, Jørgensen, M M & Varming, K 2016, ' Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication ', Journal of Circulating Biomarkers, vol. 5 . https://doi.org/10.5772/62580 |
| Popis: | Appropriate and well-documented in vitro cell-culturing systems are necessary to study the activity and biological function of extracellular vesicles (EVs). The aim of this study was to describe an experimental system, in which dynamic, vesicle-based cell communication can be investigated. A commercially available cell-culturing system was applied to study contact-independent cell communication, which separated two cell populations using a membrane with a pore size of 0.4 μm. The EV exchange characteristics between the two compartments in the culture set-up was preliminarily investigated in a cell-free set-up, and analysed using the Extracellular Vesicle (EV) Array and Nanoparticle Tracking Analysis. The application of the cell-culturing set-up was demonstrated using co-cultures of human primary cells. The effects of the relative placement of the two cell populations on the phenotype of EVs found in the cell supernatant were investigated. The results indicate that this placement can be important for the biological hypothesis that is being investigated. These observations are relevant for short ( |
| Databáze: | OpenAIRE |
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