Role for toll-like receptor 4 in TNF-alpha secretion by murine macrophages in response to polysaccharide Krestin, a Trametes versicolor mushroom extract
| Autor: | Joel W. Slaton, Jeffrey P. Novack, Daniel T. Sloper, Lisa A. Price, Cynthia A. Wenner |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Biology Proinflammatory cytokine Microbiology Mice Immune system Adjuvants Immunologic Drug Discovery Blocking antibody Animals Secretion Receptor Glucans Trametes versicolor Mice Knockout Trametes Pharmacology Biological Products Toll-like receptor Interleukin-6 Tumor Necrosis Factor-alpha General Medicine biology.organism_classification Mice Inbred C57BL Toll-Like Receptor 4 Macrophages Peritoneal TLR4 Proteoglycans Spleen |
| Zdroj: | Fitoterapia. 81:914-919 |
| ISSN: | 0367-326X |
| DOI: | 10.1016/j.fitote.2010.06.002 |
| Popis: | Woody fungi and yeast preparations show promise in cancer treatment by activating anti-tumor immune responses. Macrophages (J774A.1) were treated with PSK, Reishi extract, scleroglucan or vehicle control. Pre-incubation with TLR4 blocking antibody inhibited TNF-alpha secretion by both J774A.1 cells and primary splenocytes but had inconclusive effect on scleroglucan-induced secretion of TNF-alpha. PSK induced TNF-alpha and IL-6 secretion by wild type but not by TLR4-deficient peritoneal macrophages. We conclude that constituents from PSK act as ligands for TLR4 receptors leading to induction of TNF-alpha and IL-6 inflammatory cytokines. Receptor-mediated differences may be due to structural differences in beta glucans or non-glucan constituents. |
| Databáze: | OpenAIRE |
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