Gene expression patterns associated with human placental trophoblast differentiation
| Autor: | Wei Zhou, Shi-Wen Jiang, Jacob White Jay, Lauren M. Little, Jianhao Wang, Haibin Chen, Eseosaserea Igbinigie, Lynn Leaphart, Jinping Li |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Syncytiotrophoblasts CDC20 Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Syncytiotrophoblast Pregnancy Gene expression medicine Humans Mitosis reproductive and urinary physiology Oligonucleotide Array Sequence Analysis 030219 obstetrics & reproductive medicine Cell fusion Microarray analysis techniques Gene Expression Profiling Biochemistry (medical) Trophoblast Cell Differentiation General Medicine female genital diseases and pregnancy complications Trophoblasts Cell biology 030104 developmental biology medicine.anatomical_structure embryonic structures Female |
| Zdroj: | Clinica Chimica Acta. 495:637-645 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2018.01.012 |
| Popis: | Cell fusion is a hallmark of placental trophoblast cell differentiation and the mature syncytiotrophoblasts play essential roles for fetal-maternal exchange and production of pregnancy-related hormones. Using a well-established in vitro trophoblast differentiation model, we performed a microarray analysis on mRNA expression in trophoblast and syncytiotrophoblast cell cultures. Dramatic changes in gene expression patterns were detected during trophoblast differentiation. Real-time PCR analysis confirmed the reliability of the microarray data. As many as 3524 novel and known genes have been found to be up- or down-regulated for >2-fold. A number of cell cycle regulator including CDC6, CDC20, Cyclins B2, L1 and E2, were down-regulated in the syncytiotrophoblast, providing a mechanism for the loss of mitotic activity during trophoblast differentiation. Further characterization on the identified genes may lead to better understanding of placental patho-physiology in obstetric diseases such as preeclampsia. |
| Databáze: | OpenAIRE |
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