Sperm and Its Soluble Extract Cause Transient Increases in Intracellular Calcium Concentration and in Membrane Potential of Sea Urchin Zygotes
| Autor: | Masaki Osawa, Munehiro Kikuyama, Hideyo Kuroda, Hiroyuki Uchiyama, Yukio Hiramoto, Kazuhisa Takemoto |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Time Factors Zygote Pronase Calcium in biology Membrane Potentials Biological Factors Human fertilization Hyaline layer Species Specificity biology.animal Animals Molecular Biology Sea urchin Incubation Membrane potential biology Tissue Extracts urogenital system Sodium Cell Biology Spermatozoa Sperm Cell biology Kinetics Biochemistry Fertilization Sea Urchins embryonic structures Chromatography Gel Oocytes Calcium Female sense organs Developmental Biology |
| Zdroj: | Developmental Biology. 166:268-276 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.1994.1313 |
| Popis: | Fertilization is known to initiate a transient increase of intracellular calcium concentration and an accompanying change of membrane potential in sea urchin eggs. If the fertilization membrane and hyaline layer are removed, sperm can again enter fertilized eggs (refertilization). We have found that Ca 2+ and voltage transients were repeatedly induced in fertilized eggs during refertilization. Similar changes were also obtained by external application of a soluble extract of sperm to fertilized eggs. This sperm extract caused no changes in unfertilized eggs. The active factor in the sperm extract survives heating (100°C, 10 min) and incubation with pronase. Its molecular weight is less than 1300. |
| Databáze: | OpenAIRE |
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