Evaluation of Cobas TaqMan MTB for Direct Detection of the Mycobacterium tuberculosis Complex in Comparison with Cobas Amplicor MTB
| Autor: | Antje Voit, Vanessa Deggim, Claudia Ritter, Guido V. Bloemberg, Erik C. Böttger |
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| Přispěvatelé: | University of Zurich, Bloemberg, Guido V |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Adult Cobas taqman 610 Medicine & health Optical density Sensitivity and Specificity 2726 Microbiology (medical) Mycobacterium tuberculosis Tuberculosis diagnosis Cobas amplicor Medicine Humans Tuberculosis Prospective Studies Child Respiratory samples Bacteriological Techniques biology 10179 Institute of Medical Microbiology business.industry Mycobacteriology and Aerobic Actinomycetes Gold standard (test) biology.organism_classification Virology Molecular biology Mycobacterium tuberculosis complex Molecular Diagnostic Techniques Child Preschool 570 Life sciences business |
| Popis: | The Roche Cobas Amplicor MTB assay, recently replaced by the Roche Cobas TaqMan MTB assay, was one of the first commercially available assays for detection of the Mycobacterium tuberculosis complex based on nucleic acid amplification. We reported previously on the limited specificity of the Cobas Amplicor MTB assay, in particular for positive samples with an optical density at 660 nm (OD 660 ) of n = 838) and nonrespiratory ( n = 305) specimens. Using culture as the gold standard, we found a sensitivity of 88.4% and a specificity of 98.8% for the 838 respiratory specimens, compared to a sensitivity of 63.6% and a specificity of 94.6% for the 305 nonrespiratory specimens. We conclude that the Cobas TaqMan MTB assay is a significantly improved tool for the direct detection of M. tuberculosis DNA in clinical specimens. |
| Databáze: | OpenAIRE |
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