Evaluation of high frequency ultrasound methods and contrast agents for characterising tumor response to anti-angiogenic treatment
| Autor: | Anne Rix, Moritz Palmowski, Jessica Bzyl, Christoph Grouls, Monica Siepmann, Wiltrud Lederle, Florian F. Behrendt, Fabian Kiessling, Stanley Fokong |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
media_common.quotation_subject Contrast Media Mice Nude Angiogenesis Inhibitors Tumor response Sensitivity and Specificity Doppler imaging Mice symbols.namesake Imaging Three-Dimensional Vascularity Cell Line Tumor medicine Animals Contrast (vision) Radiology Nuclear Medicine and imaging Ultrasonography media_common Microbubbles Neovascularization Pathologic business.industry Ultrasound Reproducibility of Results General Medicine Treatment Outcome Carcinoma Squamous Cell symbols Female Radiology medicine.symptom business Nuclear medicine Doppler effect High frequency ultrasound |
| Zdroj: | European Journal of Radiology. 81:2710-2716 |
| ISSN: | 0720-048X |
| DOI: | 10.1016/j.ejrad.2011.10.004 |
| Popis: | To compare non-enhanced and contrast-enhanced high-frequency 3D Doppler ultrasound with contrast-enhanced 2D and 3D B-mode imaging for assessing tumor vascularity during antiangiogenic treatment using soft-shell and hard-shell microbubbles.Antiangiogenic therapy effects (SU11248) on vascularity of subcutaneous epidermoid-carcinoma xenografts (A431) in female CD1 nude mice were investigated longitudinally using non-enhanced and contrast-enhanced 3D Doppler at 25 MHz. Additionally, contrast-enhanced 2D and 3D B-mode scans were performed by injecting hard-shell (poly-butyl-cyanoacrylate-based) and soft-shell (phospholipid-based) microbubbles. Suitability of both contrast agents for high frequency imaging and the sensitivity of the different ultrasound methods to assess early antiangiogenic therapy effects were investigated. Ultrasound data were validated by immunohistology.Hard-shell microbubbles induced higher signal intensity changes in tumors than soft-shell microbubbles in 2D B-mode measurements (424 ± 7 vs. 169 ± 8 A.U.; p0.01). In 3D measurements, signals of soft-shell microbubbles were hardly above the background (5.48 ± 4.57 vs. 3.86 ± 2.92 A.U.), while signals from hard-shell microbubbles were sufficiently high (30.5 ± 8.06 A.U). Using hard-shell microbubbles 2D and 3D B-mode imaging depicted a significant decrease in tumor vascularity during antiangiogenic therapy from day 1 on. Using soft-shell microbubbles significant therapy effects were observed at day 4 after therapy in 2D B-mode imaging but could not be detected in the 3D mode. With non-enhanced and contrast-enhanced Doppler imaging significant differences between treated and untreated tumors were found from day 2 on.Hard-shell microbubble-enhanced 2D and 3D B-mode ultrasound achieved highest sensitivity for assessing therapy effects on tumor vascularisation and were superior to B-mode ultrasound with soft-shell microbubbles and to Doppler imaging. |
| Databáze: | OpenAIRE |
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