Symmetry and fluctuation of cell movements in neural crest-derived facial mesenchyme
| Autor: | Adrian Danescu, Joy M. Richman, Katherine Fu, Leah Edelstein-Keshet, Elisabeth G. Rens, Takashi Akazawa, Johnathan Woo, Jaspreet Rehki |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Avian embryo
media_common.quotation_subject Frontonasal Cleft Lip Cell Morphogenesis Chick Embryo Biology Eye Instability Asymmetry Mathematical analysis Fluctuating asymmetry Organ culture Mesoderm 03 medical and health sciences Craniofacial 0302 clinical medicine Cell Movement medicine Animals Live cell imaging Molecular Biology 030304 developmental biology media_common Cell Proliferation 0303 health sciences Neural tube Neural crest Brain Gene Expression Regulation Developmental Actomyosin Cleft Palate medicine.anatomical_structure Neural Crest Face Symmetry (geometry) Neuroscience Chickens 030217 neurology & neurosurgery Cell Division Developmental Biology Research Article |
| Zdroj: | Development (Cambridge, England) article-version (VoR) Version of Record |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | In the face, symmetry is established when bilateral streams of neural crest cells leave the neural tube at the same time, follow identical migration routes and then give rise to the facial prominences. However, developmental instability exists, particularly surrounding the steps of lip fusion. The causes of instability are unknown but inability to cope with developmental fluctuations are a likely cause of congenital malformations, such as non-syndromic orofacial clefts. Here, we tracked cell movements over time in the frontonasal mass, which forms the facial midline and participates in lip fusion, using live-cell imaging of chick embryos. Our mathematical examination of cell velocity vectors uncovered temporal fluctuations in several parameters, including order/disorder, symmetry/asymmetry and divergence/convergence. We found that treatment with a Rho GTPase inhibitor completely disrupted the temporal fluctuations in all measures and blocked morphogenesis. Thus, we discovered that genetic control of symmetry extends to mesenchymal cell movements and that these movements are of the type that could be perturbed in asymmetrical malformations, such as non-syndromic cleft lip. This article has an associated ‘The people behind the papers’ interview. Highlighted Article: Live imaging of the chick embryo face followed by mathematical analysis of mesenchymal cell tracks captures novel fluctuations between states of order/disorder as well as symmetry/asymmetry, revealing developmental instabilities that are part of normal morphogenesis. |
| Databáze: | OpenAIRE |
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