Direct measurement of chain order in single lipid mono- and bilayers with multiplex CARS
| Autor: | Michiel Müller, George W. H. Wurpel, Juleon M. Schins |
|---|---|
| Přispěvatelé: | Molecular Cytology (SILS, FNWI) |
| Rok vydání: | 2004 |
| Předmět: |
Quantitative Biology::Biomolecules
Physics::Biological Physics Chemistry Bilayer Phospholipid Analytical chemistry Surfaces Coatings and Films Quantitative Biology::Cell Behavior Condensed Matter::Soft Condensed Matter Quantitative Biology::Subcellular Processes chemistry.chemical_compound symbols.namesake Deuterium Phase (matter) Materials Chemistry symbols Molecule lipids (amino acids peptides and proteins) Lipid bilayer phase behavior Physical and Theoretical Chemistry Lipid bilayer Raman scattering |
| Zdroj: | The journal of Physical Chemistry. B, 108, 3400-3403. American Chemical Society |
| ISSN: | 1520-6106 |
| Popis: | We present direct measurements of the thermodynamic state of single phospholipid mono- and bilayers based on the intrinsic vibrational properties of the molecules. The increased, shot-noise limited, sensitivity of multiplex coherent anti-Stokes Raman scattering permits the acquisition of vibrational spectra of single lipid bilayers with a signal-to-noise ratio of 4 in 20 ms. The lipid chain order for both liquid-crystalline and gel phase supported lipid bilayers is determined. Using deuterated lipids, vibrational spectra can be measured selectively in either of the leaflets of an asymmetric bilayer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|