In vivo intraocular biomarkers: Changes of aqueous humor cytokines and chemokines in patients affected by uveal melanoma
| Autor: | Giulia Marchione, Raffaele Parrozzani, Luisa Frizziero, Giulia Midena, Eleonora Cosmo, Davide Londei, Edoardo Midena, Sara Trainiti |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics Uveal Neoplasms Chemokine Pathology medicine.medical_specialty medicine.medical_treatment growth chemokines Aqueous Humor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system In vivo medicine aqueous humor sample Humans 030212 general & internal medicine Melanoma biology business.industry factors Retinal General Medicine Cataract surgery Middle Aged medicine.disease proteomic analysis Pathophysiology cytokines Cytokine Cross-Sectional Studies chemistry biomarker uveal melanoma Biomarkers Case-Control Studies Chemokines Cytokines Female 030220 oncology & carcinogenesis biology.protein business |
| Popis: | Inflammatory, angiogenic, and immune processes have been associated with uveal melanoma (UM). The aim of the present study was to evaluate the presence of some specific aqueous humor (AH) soluble biomarkers in eyes affected by UM. Thirty-five eyes affected by primary UM and 35 control eyes, scheduled for cataract surgery, underwent full ophthalmic examination and AH sampling at time of surgery (brachytherapy or cataract surgery, respectively). AH samples were analyzed by means of ELISA, to detect the concentration of selected cytokines, chemokines, and growth factors. Compared with the control group, higher levels of IL-6 (P = .049), IL-8 (P = .006), RANTES (P = .008), EGF (P = .032), bFGF (P = .016), MIF (P = .007), and MCP (P = .020) were detected in eyes with UM. VEGF concentration between the two groups was statistically borderline (P = .058). Comparison between clinical characteristics and cytokine concentrations showed a positive correlation between tumor thickness and IL-8 (P = .032), and degree of serous retinal detachment and IL-6 (P = .021). UM is characterized by the presence of retinal neuroinflammatory, angiogenic, and immune biomarkers in AH. The proteomic analysis of AH could characterize UM microenvironment, allowing to better understand its pathophysiology. |
| Databáze: | OpenAIRE |
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