Radioimmunoassays of Arginine Vasopressin and Atrial Natriuretic Peptide: Application of a Common Protocol for Plasma Extraction Using Sep-Pak C18 Cartridges
| Autor: | E Mulkerrin, D A Oleesky, C Livingstone, D. Hampton, M D Penney |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
030213 general clinical medicine
medicine.medical_specialty Vasopressin Arginine Clinical Biochemistry Radioimmunoassay Neuropeptide 030209 endocrinology & metabolism Peptide hormone 03 medical and health sciences 0302 clinical medicine Atrial natriuretic peptide Internal medicine Methods medicine Humans Chemistry Elution General Medicine Silicon Dioxide Arginine Vasopressin Endocrinology Quantitative analysis (chemistry) Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 29:652-658 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/000456329202900606 |
| Popis: | A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) mixture. AVP and ANP were coeluted under positive pressure with a methanol/TFA mixture and the eluates air-dried before measurement using separate radioimmunoassays. Assay ranges for AVP and ANP were 0·12–29·5 pmol/L and 0·65–162 pmol/L, respectively, with mean recoveries (standard deviation in parentheses) for AVP of 96·4% (5·5%) at a level of 11·8 pmol/L and for ANP of 94·8% (5·9%) at a level of 32·4 pmol/L. The extraction and assay procedures were validated by observing the changes in plasma AVP and ANP concentrations in normal subjects at different stages of hydration and in elderly patients during treatment for congestive cardiac failure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|