Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product 'Btana'
| Autor: | Carine Nezer, Serge Hiligsman, Georges Daube, Kihal Mabrouk, Bernard Taminiau, Abdelkader Abekhti |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Traditional food Biotechnologie Pichia subpelliculosa Sciences de l'ingénieur Microbiology Esterase 03 medical and health sciences chemistry.chemical_compound Food Preservation Yeasts RNA Ribosomal 28S Ethanol fuel Food science Lactic Acid DNA Fungal Phylogeny 030304 developmental biology 0303 health sciences Ascomycota biology Ethanol 030306 microbiology Phylum Dates Fungi Pyrosequencing Biodiversity biology.organism_classification Lactic acid chemistry Saccharomycetales Fermentation Metabarcoding Fermented Foods Mycobiome Btana |
| Zdroj: | International microbiology |
| Popis: | The study highlights the fungal diversity of the traditional Algerian date’s product “Btana” prepared with direct Btana method (DBM) and undirect Btana method (UBM). Btana fungal populations were analyzed through 28S metabarcoding. Data treatment resulted in 122,997 reads representing three Phyla in which 76% reads (46 OTUs) belong to Ascomycota phylum. Zygosaccharomycesrouxii was the most prevailed species accounting for 35.40% of the total population. Similarity percentage analysis revealed a low level of resemblance in species in each of the two Btana types (DBM: 17.26%, UBM: 16.87). According to HPLC analysis, lactate was detected in nine samples within a range of 0.87-23.06 g/100g. Culture plating and subsequent D1/D2 domain of 28s DNA analysis showed the prevalence of Z. rouxii. Fermentation of non-renewed date medium revealed a high ethanol production (21.31 ± 2.89 g/100g) by Lachanceathermotolerans and 5.87 g/100g of lactates by Kluyveromycesdelphensis. Enzymatic assay revealed a high esterase (C4) and naphtol-AS-BI-phosphohydrolase activity by L. thermotolerans, K. delphensis, and Pichiasubpelliculosa, while a high level of α-fucosidase was recorded for L. thermotolerans and P. subpelliculosa. The current results demonstrated that the traditional date product Btana is a promising source for yeasts useful in production of value-added products like bioethanol and lactic acid using low-income date cultivars. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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