Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
| Autor: | Slavica Dmitrović, Snežana Zdravković-Korać, Martin Raspor, Mirka Djordjević, Vuk Maksimović, Suzana Živković, Nevena Mitić, Mariana Stanišić, Tatjana Djordjević, Dijana Krstić-Milošević, Slavica Ninković, Radomirka Nikolić |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
food.ingredient Physiology Agrobacterium Plant Science 01 natural sciences Plant Roots Pheromones 03 medical and health sciences chemistry.chemical_compound Chenopodium food Transformation Genetic Gene Expression Regulation Plant Culture Techniques Botany Allelopathy 030304 developmental biology 0303 health sciences Growth medium biology Genetic Variation biology.organism_classification Plants Genetically Modified Clone Cells Culture Media Plant Leaves chemistry Seedling Germination Seedlings Agronomy and Crop Science Cotyledon 010606 plant biology & botany Explant culture |
| Zdroj: | Journal of Plant Physiology |
| ISSN: | 1618-1328 |
| Popis: | We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved. |
| Databáze: | OpenAIRE |
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