Landscapes and dynamic diversifications of B-cell receptor repertoires in COVID-19 patients
Autor: | Penghui Yang, Xiaoshan Wang, Yuhuan Gong, George F. Gao, William J. Liu, Jin Yan, Peipei Liu, Ziqian Xu, Beiwei Ye, Longlong Wang, Ning Zhang, Naibo Yang, Haitao Xiang, Xinyang Li, Zhaohai Wang, Xiaopan Liu, Longqi Liu, Guizhen Wu, Ying Gu, Shaogeng Zhang, Linxiang Yu, Chen Zhu, Fanping Meng, Yingze Zhao, Xiaoju Yuan, Meiniang Wang, Wei Zhang, Pengyan Wang, Chengrong Bian, Kai Gao, Yi Shi, Liang Wu, Changqing Bai, Xun Xu, Haixi Sun, Yuhai Bi, Lei Tian |
---|---|
Rok vydání: | 2022 |
Předmět: |
Adult
Male Lineage (genetic) Adolescent Immunology B-cell receptor Receptors Antigen B-Cell BCR B-cell receptor Antibodies Viral Virus Immune system medicine Humans Immunology and Allergy Gene B cell COVID-19 coronavirus disease 2019 Aged 80 and over B-Lymphocytes SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 biology SARS-CoV-2 Repertoire COVID-19 B-cell receptor repertoire General Medicine Middle Aged Antibodies Neutralizing Clonal expansion medicine.anatomical_structure PBMC peripheral blood mononuclear cells SHM somatic hypermutation biology.protein Female Antibody Immunoglobulin Heavy Chains CDR3 complementarity determining region 3 IGH immunoglobin heavy chain Research Article |
Zdroj: | Human Immunology |
ISSN: | 0198-8859 |
Popis: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the pandemic of coronavirus disease 2019 (COVID-19). Great international efforts have been put into the development of prophylactic vaccines and neutralizing antibodies. However, the knowledge about the B cell immune response induced by the SARS-CoV-2 virus is still limited. Here, we report a comprehensive characterization of the dynamics of immunoglobin heavy chain (IGH) repertoire in COVID-19 patients. By using next-generation sequencing technology, we examined the temporal changes in the landscape of the patient's immunological status and found dramatic changes in the IGH within the patient's immune system after the onset of COVID-19 symptoms. Although different patients have distinct immune responses to SARS-CoV-2 infection, by employing clonotype overlap, lineage expansion, and clonotype network analyses, we observed a higher clonotype overlap and substantial lineage expansion of B cell clones 2-3 weeks after the onset of illness, which is of great importance to B-cell immune responses. Meanwhile, for preferences of V gene usage during SARS-CoV-2 infection, IGHV3-74 and IGHV4-34, and IGHV4-39 in COVID-19 patients were more abundant than those of healthy controls. Overall, we present an immunological resource for SARS-CoV-2 that could promote both therapeutic development as well as mechanistic research. |
Databáze: | OpenAIRE |
Externí odkaz: |