Herpes simplex virus type 1 polypeptide ICP4 bends DNA
| Autor: | Margaret Elliott, Roger D. Everett, Joe DiDonato, Mark T. Muller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
HMG-box
viruses Restriction Mapping medicine.disease_cause Single-stranded binding protein Immediate-Early Proteins chemistry.chemical_compound Genetics medicine Simplexvirus Viral Regulatory and Accessory Proteins Binding site Binding Sites biology Binding protein DNA-binding domain DNA Virology Molecular biology DNA binding site DNA-Binding Proteins Herpes simplex virus chemistry biology.protein Nucleic Acid Conformation |
| ISSN: | 0305-1048 |
| Popis: | ICP4, the major regulatory polypeptide of herpes\ud simplex virus type 1, is expressed at the earliest stages\ud of virus infection and is required for the activation of\ud transcription from the majority of viral promoters. It is\ud a DNA binding protein which specifically recognises bipartite sites related to the sequence ATCGTnnnnnCGG.\ud In this report we show that both partially purified ICP4,\ud and its isolated DNA binding domain, bend DNA at\ud occupied binding sites. The apparent angles of bend\ud at two different binding sites were very similar and in\ud both cases the centre of the bend was very close to\ud the binding site sequence. |
| Databáze: | OpenAIRE |
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