Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells
| Autor: | Hisato Saitoh, Takashi Sakugawa, Ken Ichi Yano, Tsubasa Koga, Keiko Morotomi-Yano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell biology Neutrophils Cell Biophysics lcsh:Medicine Apoptosis HL-60 Cells Extracellular Traps Article Histones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Citrulline Extracellular medicine Humans lcsh:Science Multidisciplinary medicine.diagnostic_test biology lcsh:R Cell Differentiation Neutrophil extracellular traps DNA Chromatin Electric Stimulation Histone citrullination 030104 developmental biology Histone medicine.anatomical_structure chemistry biology.protein lcsh:Q Citrullination Leukopoiesis 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Nanosecond pulsed electric fields (nsPEFs) have gained attention as a novel physical stimulus for life sciences. Although cancer therapy is currently their promising application, nsPEFs have further potential owing to their ability to elicit various cellular responses. This study aimed to explore stimulatory actions of nsPEFs, and we used HL-60 cells that were differentiated into neutrophils under cultured conditions. Exposure of neutrophil-differentiated HL-60 cells to nsPEFs led to the extracellular release of chromosomal DNA, which appears to be equivalent to neutrophil extracellular traps (NETs) that serve as a host defense mechanism against pathogens. Fluorometric measurement of extracellular DNA showed that DNA extrusion was rapidly induced after nsPEF exposure and increased over time. Western blot analysis demonstrated that nsPEFs induced histone citrullination that is the hydrolytic conversion of arginine to citrulline on histones and facilitates chromatin decondensation. DNA extrusion and histone citrullination by nsPEFs were cell type-specific and Ca2+-dependent events. Taken together, these observations suggest that nsPEFs drive the mechanism for neutrophil-specific immune response without infection, highlighting a novel aspect of nsPEFs as a physical stimulus. |
| Databáze: | OpenAIRE |
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