Generation of monoclonal antibodies to recombinant vascular endothelial growth factor

Autor:	Vladimir P. Baklaushev, I. A. Ryabukhin, N. E. Volgina, N. V. Ivanova, N. F. Grinenko, A. V. Leopol’d, Vladimir P. Chekhonin, A. A. Korchagina, S. A. Shein, Olga I. Gurina
Rok vydání:	2012
Předmět:	Vascular Endothelial Growth Factor A medicine.drug_class chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Monoclonal antibody General Biochemistry Genetics and Molecular Biology law.invention chemistry.chemical_compound Mice law Cell Line Tumor medicine Animals Cloning Mice Inbred BALB C biology Antibodies Monoclonal General Medicine Molecular biology Immunohistochemistry Recombinant Vascular Endothelial Growth Factor Vascular endothelial growth factor Immunization chemistry Monoclonal biology.protein Recombinant DNA Female Antibody
Zdroj:	Bulletin of experimental biology and medicine. 153(1)
ISSN:	1573-8221
Popis:	Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c4a13b79bf7e9c21d3db93d48904852 https://pubmed.ncbi.nlm.nih.gov/22808513 Zobrazit plný text záznamu Full text from SpringerLink